RRC ID |
6574
|
著者 |
Sato K, Koyama T, Shichiri M.
|
タイトル |
Biosynthesis and secretion of salusin-alpha from human cells.
|
ジャーナル |
Peptides
|
Abstract |
Salusins originally identified using bioinformatics analyses have been shown to act on the cardiovascular and endocrine systems. Although the hypotensive activity of salusin-alpha is limited, it exerts a significant anti-atherosclerotic effect via suppression of foam cell formation in human monocyte-derived macrophages by down-regulating acyl-CoA:cholesterol acyltransferase-1. Furthermore, serum salusin-alpha levels show a close negative correlation with the severity of atherosclerotic diseases. However, biosynthesis and secretion of salusin-alpha peptide from cultured mammalian cells have not been demonstrated to date. We examined the expression, synthesis and release of salusin-alpha in human-derived cell lines. Preprosalusin mRNA and protein were detected ubiquitously in all cells tested, whereas the processing of preprosalusin into salusin-alpha peptide is dependent upon each cell type. Immunohistochemical study revealed the most abundant salusin-alpha-like immunoreactivity to be present in HeLa cells which released salusin-alpha-like immunoreactivity into the culture supernatant. Analysis of extracted conditioned media from HeLa cells by reverse-phase high performance liquid chromatography coupled with radioimmunoassay detection revealed a single immunoreactive component that co-eluted with authentic salusin-alpha. These results present the first evidence that salusin-alpha is biosynthesized and released from human-derived cells.
|
巻・号 |
29(12)
|
ページ |
2203-7
|
公開日 |
2008-12-1
|
DOI |
10.1016/j.peptides.2008.08.015
|
PII |
S0196-9781(08)00360-4
|
PMID |
18804130
|
MeSH |
Base Sequence
Cell Line
Humans
Intercellular Signaling Peptides and Proteins / biosynthesis*
Intercellular Signaling Peptides and Proteins / metabolism
Molecular Sequence Data
RNA, Messenger / metabolism*
|
IF |
2.843
|
引用数 |
14
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
MKN1(RCB1003) |