| RRC ID |
51419
|
| 著者 |
González-Huici V, Wang B, Gartner A.
|
| タイトル |
A Role for the Nonsense-Mediated mRNA Decay Pathway in Maintaining Genome Stability in Caenorhabditis elegans.
|
| ジャーナル |
Genetics
|
| Abstract |
Ionizing radiation (IR) is commonly used in cancer therapy and is a main source of DNA double-strand breaks (DSBs), one of the most toxic forms of DNA damage. We have used Caenorhabditis elegans as an invertebrate model to identify novel factors required for repair of DNA damage inflicted by IR. We have performed an unbiased genetic screen, finding that smg-1 mutations confer strong hyper-sensitivity to IR. SMG-1 is a phosphoinositide-3 kinase (PI3K) involved in mediating nonsense-mediated mRNA decay (NMD) of transcripts containing premature stop codons and related to the ATM and ATR kinases which are at the apex of DNA damage signaling pathways. Hyper-sensitivity to IR also occurs when other genes mediating NMD are mutated. The hyper-sensitivity to bleomycin, a drug known to induce DSBs, further supports that NMD pathway mutants are defective in DSB repair. Hyper-sensitivity was not observed upon treatment with alkylating agents or UV irradiation. We show that SMG-1 mainly acts in mitotically dividing germ cells, and during late embryonic and larval development. Based on epistasis experiments, SMG-1 does not appear to act in any of the three major pathways known to mend DNA DSBs, namely homologous recombination (HR), nonhomologous end-joining (NHEJ), and microhomology-mediated end-joining (MMEJ). We speculate that SMG-1 kinase activity could be activated following DNA damage to phosphorylate specific DNA repair proteins and/or that NMD inactivation may lead to aberrant mRNAs leading to synthesis of malfunctioning DNA repair proteins.
|
| 巻・号 |
206(4)
|
| ページ |
1853-1864
|
| 公開日 |
2017-8-1
|
| DOI |
10.1534/genetics.117.203414
|
| PII |
genetics.117.203414
|
| PMID |
28634159
|
| PMC |
PMC5560793
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / radiation effects
Caenorhabditis elegans Proteins / genetics
DNA Breaks, Double-Stranded
DNA Damage
DNA Repair
Epistasis, Genetic
Genomic Instability*
Homologous Recombination
Mitosis
Nonsense Mediated mRNA Decay*
Protein Serine-Threonine Kinases / genetics
Radiation, Ionizing
|
| IF |
4.015
|
| 引用数 |
3
|
| リソース情報 |
| 線虫 |
tm1145
tm2026
tm2842
tm2940 |
