論文 - 詳細
| RRC ID | 51730 |
|---|---|
| 著者 | Konagaya S, Iwata H. |
| タイトル | Reproducible preparation of spheroids of pancreatic hormone positive cells from human iPS cells: An in vitro study. |
| ジャーナル | Biochim Biophys Acta |
| Abstract |
BACKGROUND:Transplantation of islets of Langerhans is regarded as a promising therapy for type 1 diabetes. A large number of β-cells are required for the treatment of human type 1 diabetes. Pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells, have been considered as new sources for cell replacement therapy. METHODS:Cell aggregates were prepared from human iPS cells using agarose microwell plates and differentiated into pancreatic endocrine cells by changing the culture media with different additives. RESULTS:After 20days of culture, approximately 30% of cells in aggregates were positive for C-peptide. After another 14days in culture, the cells gained an ability to alter C-peptide release in response to changes in the glucose concentration. CONCLUSIONS:Uniform aggregates of human iPSCs were easily prepared on agarose microwell plates and efficiently differentiated into the pancreatic endocrine lineage. Thus, aggregate culture is a suitable method for preparing islet-like aggregates from human iPSCs. GENERAL SIGNIFICANCE:Our results indicate that the microwell plate is suitable for scaling up the preparation of pancreatic endocrine cells from human iPS cells in a robotic system. |
| 巻・号 | 1860(9) |
| ページ | 2008-16 |
| 公開日 | 2016-9-1 |
| DOI | 10.1016/j.bbagen.2016.05.012 |
| PII | S0304-4165(16)30154-4 |
| PMID | 27180174 |
| MeSH | C-Peptide / metabolism Cell Differentiation / physiology Cell Lineage / physiology Cells, Cultured Diabetes Mellitus, Type 1 / metabolism Embryonic Stem Cells / cytology Embryonic Stem Cells / metabolism Endocrine Cells / cytology Endocrine Cells / metabolism Glucose Humans Induced Pluripotent Stem Cells / cytology* Induced Pluripotent Stem Cells / metabolism Insulin / metabolism Insulin-Secreting Cells / cytology Insulin-Secreting Cells / metabolism Pancreatic Hormones / metabolism* Pluripotent Stem Cells / cytology Pluripotent Stem Cells / metabolism |
| IF | 3.411 |
| 引用数 | 11 |
| リソース情報 | |
| ヒト・動物細胞 | 454E2(HPS0077) HiPS-RIKEN-2A(HPS0009) |