RRC ID 51881
著者 Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.
タイトル A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.
ジャーナル Biochim Biophys Acta
Abstract N-Acylethanolamine acid amidase (NAAA) is a lysosomal enzyme, hydrolyzing various bioactive N-acylethanolamines with a preference for palmitoylethanolamide. Human NAAA mRNA was previously reported to consist of multiple 3'-end splice variants. However, their tissue distributions and roles have not been examined yet. In the present study, we first identified four major splice variants (tentatively referred to as a1, a2, b2, and c2) in a human prostate cancer cell line LNCaP, which were composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively. We next developed quantitative polymerase chain reaction methods to individually quantify these NAAA variants as well as collectively measure all the variants. Among various human prostate cancer cells, the total levels of NAAA mRNAs in androgen-sensitive cells like LNCaP were higher than those in androgen-insensitive cells. In all of these prostate cells and other human cells, variants a1 and b2 showed the highest and lowest expression levels, respectively, among the four variants. Interestingly, ratios of the four variants were different by cell type. Variants a1 and a2 encoded the same full-length NAAA protein, which was catalytically active, while b2 and c2 were translated to C-terminally truncated proteins. As expressed in HEK293 cells these truncated forms were detected as catalytically inactive precursor proteins, but not as mature forms. These results revealed wide distribution of multiple variants of NAAA mRNA in various human cells and suggested that the proteins from some variants are catalytically inactive.
巻・号 1861(12 Pt A)
ページ 1951-1958
公開日 2016-12-1
DOI 10.1016/j.bbalip.2016.09.018
PII S1388-1981(16)30264-5
PMID 27693242
MeSH Amidohydrolases / genetics* Ethanolamines / metabolism Evaluation Studies as Topic Exons / genetics Genetic Variation / genetics* HEK293 Cells HeLa Cells Humans MCF-7 Cells Male Prostatic Neoplasms / genetics* Prostatic Neoplasms / metabolism RNA Splicing / genetics* RNA, Messenger / genetics
IF 3.411
引用数 3
リソース情報
ヒト・動物細胞 LNCap.FGC(RCB2144) DU145(RCB2143) PC-3(RCB2145)