RRC ID 48134
著者 Kim DH, Singh P, Farh Mel-A, Kim YJ, Nguyen NL, Lee HA, Yang DC.
タイトル Flavobacterium panacis sp. nov., isolated from rhizosphere of Panax ginseng.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel bacterial strain, designated DCY106(T), was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY106(T) is Gram-negative, yellow-pigmented, non-flagellate, motile, non-spore-forming, rod-shaped, and strictly aerobic. The strain grows optimally at 25-30 °C and pH 6.5-7.5. Phylogenetically, strain DCY106(T) is closely related to Flavobacterium arsenitoxidans KCTC 22507(T) (98.41 %), followed by Flavobacterium cutihirudini LMG 26922(T) (97.67 %), Flavobacterium nitrogenifigens LMG 28694(T) (97.59 %), Flexibacter auranticus LMG 3987(T) (97.38 %), Flavobacterium defluvi KCTC 12612(T) (97.21 %) and Flavobacterium chilense LMG 26360(T) (97.05 %). The 16S rRNA gene sequence similarities to all other Flavobacterium species were below 97 %. The DNA G+C content of strain DCY106(T) is 34.2 mol% and the DNA-DNA relatedness between strain DCY106(T) and F. cutihirudini LMG 26922(T), F. auranticus LMG 3987(T), F. defluvi KCTC 12612(T) and F. chilense LMG 26360(T) were below 40.0 %. The menaquinone of the type MK-6 was found to be the predominant respiratory quinone. The major polar lipids were identified as phosphatidylethanolamine, phosphatidylserine, two unidentified aminolipids (APL1, APL6) and one unidentified lipid L2. C15:0, iso-C15:0 and summed feature 3 (iso-C15:0 2OH/C16:1 ω7c) were identified as the major fatty acids present in DCY106(T). The results of physiological and biochemical tests allowed strain DCY106(T) to be differentiated phenotypically from other recognized species belonging to the genus Flavobacterium. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Flavobacterium panacis sp. nov. is proposed with the type strain designated as DCY106(T) (= JCM 31468(T)= KCTC 42747(T)).
巻・号 109(9)
ページ 1199-208
公開日 2016-9-1
DOI 10.1007/s10482-016-0720-7
PII 10.1007/s10482-016-0720-7
PMID 27357576
MeSH Base Composition Cell Wall / metabolism DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / metabolism Flavobacterium / classification* Flavobacterium / genetics Flavobacterium / isolation & purification* Flavobacterium / physiology Panax / microbiology* Phosphatidylethanolamines / metabolism Phosphatidylserines / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Republic of Korea Rhizosphere Soil Microbiology Species Specificity Vitamin K 2 / metabolism
IF 2.674
引用数 3
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 31468