RRC ID 48147
著者 Han C, Liu C, Zhao J, Guo L, Lu C, Li J, Jia F, Wang X, Xiang W.
タイトル Microbispora camponoti sp. nov., a novel actinomycete isolated from the cuticle of Camponotus japonicus Mayr.
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel actinomycete, designated strain 2C-HV3(T), was isolated from the cuticle of Camponotus japonicus Mayr collected from Harbin, Heilongjiang province, north China and characterised using a polyphasic approach. The 16S rRNA gene sequence of strain 2C-HV3(T) showed that it has high sequence similarities with Microbispora bryophytorum NEAU-TX2-2(T) (99.9 %), Microbispora amethystogenes JCM 3021(T) (98.9 %) and Microbispora rosea subsp. rosea JCM 3006(T) (98.6 %). Phylogenetic analysis based on 16S rRNA and gyrB gene sequences demonstrated that strain 2C-HV3(T) clusters with M. bryophytorum NEAU-TX2-2(T) using two tree-making algorithms. Moreover, key morphological and chemotaxonomic properties also confirmed the affiliation of strain 2C-HV3(T) to the genus Microbispora. Longitudinal paired spores were observed to be born on short sporophores branching from the aerial hyphae. The cell wall was found to contain meso-diaminopimelic acid as the diagnostic diamino acid; madurose was found in the whole cell hydrolysate. The polar lipid profile was found to consist of diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, ninhydrin-positive glycophospholipids, an unidentified phospholipid and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H2) and MK-9(H4). The major fatty acids were identified as 10-methyl C17:0 and iso-C16:0. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain 2C-HV3(T) can be distinguished from its closely related relatives. Consequently, it is proposed that strain 2C-HV3(T) represents a new species of the genus Microbispora, for which the name Microbispora camponoti sp. nov. is proposed. The type strain is 2C-HV3(T) (=CGMCC 4.7281(T) = DSM 100527(T)).
巻・号 109(2)
ページ 215-23
公開日 2016-2-1
DOI 10.1007/s10482-015-0625-x
PII 10.1007/s10482-015-0625-x
PMID 26589683
MeSH Actinomycetales / classification Actinomycetales / genetics Actinomycetales / isolation & purification* Actinomycetales / metabolism Animal Structures / microbiology* Animals Ants / microbiology* China DNA, Bacterial / genetics DNA, Ribosomal / genetics Fatty Acids / chemistry Fatty Acids / metabolism Molecular Sequence Data Phylogeny
IF 2.674
引用数 10
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 3021 JCM 3006