RRC ID 36171
著者 Martín-Bermudo MD, Bardet PL, Bellaïche Y, Malartre M.
タイトル The vav oncogene antagonises EGFR signalling and regulates adherens junction dynamics during Drosophila eye development.
ジャーナル Development
Abstract Organ shaping and patterning depends on the coordinated regulation of multiple processes. The Drosophila compound eye provides an excellent model to study the coordination of cell fate and cell positioning during morphogenesis. Here, we find that loss of vav oncogene function during eye development is associated with a disorganised retina characterised by the presence of additional cells of all types. We demonstrate that these defects result from two distinct roles of Vav. First, and in contrast to its well-established role as a positive effector of the EGF receptor (EGFR), we show that readouts of the EGFR pathway are upregulated in vav mutant larval eye disc and pupal retina, indicating that Vav antagonises EGFR signalling during eye development. Accordingly, decreasing EGFR signalling in vav mutant eyes restores retinal organisation and rescues most vav mutant phenotypes. Second, using live imaging in the pupal retina, we observe that vav mutant cells do not form stable adherens junctions, causing various defects, such as recruitment of extra primary pigment cells. In agreement with this role in junction dynamics, we observe that these phenotypes can be exacerbated by lowering DE-Cadherin or Cindr levels. Taken together, our findings establish that Vav acts at multiple times during eye development to prevent excessive cell recruitment by limiting EGFR signalling and by regulating junction dynamics to ensure the correct patterning and morphogenesis of the Drosophila eye.
巻・号 142(8)
ページ 1492-501
公開日 2015-4-15
DOI 10.1242/dev.110585
PII dev.110585
PMID 25813543
MeSH Adherens Junctions / metabolism* Animals Body Patterning / genetics Body Patterning / physiology Cadherins / genetics Cadherins / metabolism Drosophila Drosophila Proteins / genetics Drosophila Proteins / metabolism ErbB Receptors / genetics ErbB Receptors / metabolism* Eye / embryology Eye / metabolism* Microfilament Proteins / genetics Microfilament Proteins / metabolism Morphogenesis / genetics Morphogenesis / physiology Proto-Oncogene Proteins c-vav / genetics Proto-Oncogene Proteins c-vav / metabolism* Signal Transduction / genetics Signal Transduction / physiology
IF 5.611
引用数 6
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ DGRC#111794