RRC ID 41042
著者 Li Y, Wang T, Piao CG, Wang LF, Tian GZ, Zhu TH, Guo MW.
タイトル Lampropedia puyangensis sp. nov., isolated from symptomatic bark of Populus × euramericana canker and emended description of Lampropedia hyalina (Ehrenberg 1832) Lee et al. 2004.
ジャーナル Antonie Van Leeuwenhoek
Abstract A Gram-stain negative, Neisser-stain negative, aerobic, non-motile, non-spore-forming, slimy, glossy bacterial strain with single or clustered coccoid cells and white colony colour, designated as 2-bin(T), was isolated from cankered bark tissue of Populus × euramericana. The strain was found to grow at 15-40 °C and pH 5-10, with an optimum of 30 °C and pH 8.0. The strain was found to be negative with respect to catalase and positive for oxidase activity, nitrate reduction and Voges-Proskauer reaction. Analysis of 16S rRNA gene sequence data indicated that the isolate belongs to the genus Lampropedia, having sequence similarity of 96.24 % with Lampropedia hyalina ATCC11041(T). DNA-DNA relatedness of strain 2-bin(T) with L. hyalina JCM 21380(T) was 26.7 ± 4.6 %. The DNA G+C content of strain 2-bin(T) was determined to be 57 % and the major cellular fatty acids were identified as C16:0, C16:1 ω7c/C16:1 ω6c and C18:1 ω7c. The polar lipid profile of strain 2-bin(T) was found to contain diphosphatidylglycerol, a glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, phosphatidylmonomethylethanolamine and three unidentified lipids (L1, L2, L3). Based on molecular data and physiological and biochemical characteristics, strain 2-bin(T) is considered to represent a novel species in the genus Lampropedia, for which the name Lampropedia puyangensis sp. nov. is proposed. The type strain is 2-bin(T) (= CFCC 10925(T) = KCTC 32235(T)).
巻・号 108(2)
ページ 321-8
公開日 2015-8-1
DOI 10.1007/s10482-015-0483-6
PMID 25991383
MeSH Aerobiosis Bacterial Typing Techniques Base Composition Cluster Analysis Comamonadaceae / classification* Comamonadaceae / genetics Comamonadaceae / isolation & purification* Comamonadaceae / physiology Cytosol / chemistry DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Glycolipids / analysis Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Phospholipids / analysis Phylogeny Plant Bark / microbiology* Plant Diseases / microbiology* Populus / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Temperature
IF 2.674
引用数 6
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 21380