RRC ID 12335
著者 Takase T, Nishiyama Y, Tanihigashi H, Ogura Y, Miyazaki Y, Yamada Y, Kiyosue T.
タイトル LOV KELCH PROTEIN2 and ZEITLUPE repress Arabidopsis photoperiodic flowering under non-inductive conditions, dependent on FLAVIN-BINDING KELCH REPEAT F-BOX1.
ジャーナル Plant J
Abstract LOV KELCH PROTEIN2 (LKP2), ZEITLUPE (ZTL)/LOV KELCH PROTEIN1 (LKP1) and FLAVIN-BINDING KELCH REPEAT F-BOX1 (FKF1) constitute a family of Arabidopsis F-box proteins that regulate the circadian clock. Over-expression of LKP2 or ZTL causes arrhythmicity of multiple clock outputs under constant light and in constant darkness. Here, we show the significance of LKP2 and ZTL in the photoperiodic control of flowering time in Arabidopsis. In plants over-expressing LKP2, CO and FT expression was down-regulated under long-day conditions. LKP2 and ZTL physically interacted with FKF1, which was recruited from the nucleus into cytosolic speckles. LKP2 and ZTL inhibited the interaction of FKF1 with CYCLING DOF FACTOR 1, a ubiquitination substrate for FKF1 that is localized in the nucleus. The Kelch repeat regions of LKP2 and ZTL were sufficient for their physical interaction with FKF1 and translocation of FKF1 to the cytoplasm. Over-expression of LKP2 Kelch repeats induced late flowering under long-day conditions. lkp2 ztl double mutant plants flowered earlier than wild-type plants under short-day (non-inductive) conditions, and both CO and FT expression levels were up-regulated in the double mutant plants. The early flowering of lkp2 ztl was dependent on FKF1. LKP2, ZTL or both affected the accumulation of FKF1 protein during the early light period. These results indicate that an important role of LKP2 and ZTL in the photoperiodic pathway is repression of flowering under non-inductive conditions, and this is dependent on FKF1.
巻・号 67(4)
ページ 608-21
公開日 2011-8-1
DOI 10.1111/j.1365-313X.2011.04618.x
PMID 21518052
MeSH Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / physiology* Arabidopsis / ultrastructure* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cell Nucleus / metabolism Cytosol / metabolism Down-Regulation F-Box Proteins / genetics F-Box Proteins / metabolism Flowers / genetics Flowers / physiology Gene Expression Regulation, Plant / physiology* Genetic Complementation Test Phenotype Photoperiod Plant Leaves / genetics Plant Leaves / physiology Plant Shoots / genetics Plant Shoots / physiology Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / physiology Plants, Genetically Modified / ultrastructure Sequence Deletion
IF 6.141
引用数 44
WOS 分野 PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 rpc00008