RRC ID 33936
著者 Shiraishi H.
タイトル Association of heterotrophic bacteria with aggregated Arthrospira platensis exopolysaccharides: implications in the induction of axenic cultures.
ジャーナル Biosci Biotechnol Biochem
Abstract Inducing an axenic culture of the edible cyanobacterium Arthrospira (Spirulina) platensis using differential filtration alone is never successful; thus, it has been thought that, in non-axenic cultures, a portion of contaminating bacteria is strongly associated with Arthrospira cells. However, examination of the behavior of these bacteria during filtration revealed that they were not associated with Arthrospira cells but with aggregates of exopolysaccharides present in the medium away from the Arthrospira cells. Based on this finding, a rapid and reliable method for preparing axenic trichomes of A. platensis was established. After verifying the axenicity of the resulting trichomes on enriched agar plates, they were individually transferred to fresh sterile medium using a handmade tool, a microtrowel, to produce axenic cultures. With this technique, axenic cultures of various A. platensis strains were successfully produced. The technique described in this study is potentially applicable to a wider range of filamentous cyanobacteria.
巻・号 79(2)
ページ 331-41
公開日 2015-1-1
DOI 10.1080/09168451.2014.972333
PMID 25333502
MeSH Axenic Culture / methods* Extracellular Space / metabolism Filtration Heterotrophic Processes* Polysaccharides, Bacterial / metabolism* Spirulina / cytology Spirulina / growth & development* Spirulina / metabolism*
IF 1.516
引用数 9
WOS 分野 FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CHEMISTRY, APPLIED
リソース情報
藻類 NIES-39 NIES-46