RRC ID 41311
著者 Tomoike F, Tsunetou A, Kim K, Nakagawa N, Kuramitsu S, Masui R.
タイトル A putative adenosine kinase family protein possesses adenosine diphosphatase activity.
ジャーナル Biosci Biotechnol Biochem
Abstract Adenosine kinase is a potential target for development of new types of drugs. The COG1839 family has been defined as "adenosine-specific kinase" family based on structural analysis and the adenosine-binding ability of a family member, PAE2307. However, there has been no experimental evidence with regard to the enzymatic function of this protein family. Here we measured the enzymatic activity of TTHA1091, a COG1839 family protein from Thermus thermophilus HB8. The phosphorylation of adenosine by TTHA1091 was undetectable when ATP or ADP were used as phosphate donor. However, the degradation of ADP to AMP was detected, indicating that this protein possessed adenosine diphosphatase (ADPase) activity. The (ADPase) activity was inhibited by divalent cations and was specific to ADP and CDP. Thus, this study provides the first experimental evidence for the enzymatic function of the "adenosine-specific kinase" family and suggests a need to reexamine its functional annotation.
巻・号 80(11)
ページ 2138-2143
公開日 2016-11-1
DOI 10.1080/09168451.2016.1214532
PII 10.1080/09168451.2016.1214532
PMID 27484886
IF 1.516
引用数 1
リソース情報
遺伝子材料 Thermus thermophilus expression plasmid TEx02C04 (THR000852)