RRC ID 48790
著者 Oh K, Matsumoto T, Hoshi T, Yoshizawa Y.
タイトル In vitro and in vivo evidence for the inhibition of brassinosteroid synthesis by propiconazole through interference with side chain hydroxylation.
ジャーナル Plant Signal Behav
Abstract We carried out the biochemical evaluation of the target site of propiconazole in BR biosynthesis. Applying BR biosynthesis intermediates to Arabidopsis seedlings grown in the presence of propiconazole under dark condition, we found that the target site of propiconazole in BR biosynthesis can be identified among the C22 and C23 side chain hydroxylation steps from campestanol to teasterone. Using differential spectra techniques to determine the binding affinity of propiconazole to CYP90D1, which is responsible for C23 hydroxylation of BR, we found that propiconazole induced typical type II binding spectra in response to purified recombinant CYP90D1 and the Kd value was found approximately 0.76 μM.
巻・号 11(5)
ページ e1158372
公開日 2016-5-3
DOI 10.1080/15592324.2016.1158372
PMID 26987039
PMC PMC4977458
MeSH Arabidopsis / drug effects Arabidopsis / enzymology Arabidopsis / metabolism* Arabidopsis Proteins / metabolism Brassinosteroids / biosynthesis* Cytochrome P-450 Enzyme System / metabolism Hydroxylation / drug effects Kinetics Protein Binding / drug effects Recombinant Proteins / metabolism Seedlings / drug effects Seedlings / metabolism Triazoles / chemistry Triazoles / pharmacology*
IF 1.671
引用数 4
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY PLANT SCIENCES
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda13118