RRC ID 49382
著者 Berlendis S, Ranchou-Peyruse M, Fardeau ML, Lascourrèges JF, Joseph M, Ollivier B, Aüllo T, Dequidt D, Magot M, Ranchou-Peyruse A.
タイトル Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov., isolated from a deep natural gas storage aquifer.
ジャーナル Int J Syst Evol Microbiol
Abstract Two novel strictly anaerobic bacteria, strains Bs105T and Bs107T, were isolated from a deep aquifer-derived hydrocarbonoclastic community. The cells were rod-shaped, not motile and had terminal spores. Phylogenetic affiliation and physiological properties revealed that these isolates belong to two novel species of the genus Desulfotomaculum. Optimal growth temperatures for strains Bs105T and Bs107T were 42 and 45 °C, respectively. The estimated G+C content of the genomic DNA was 42.9 and 48.7 mol%. For both strains, the major cellular fatty acid was palmitate (C16 : 0). Specific carbon fatty acid signatures of Gram-positive bacteria (iso-C17 : 0) and sulfate-reducing bacteria (C17 : 0cyc) were also detected. An insertion was revealed in one of the two 16S rRNA gene copies harboured by strain Bs107T. Similar insertions have previously been highlighted among moderately thermophilic species of the genus Desulfotomaculum. Both strains shared the ability to oxidize aromatic acids (Bs105T: hydroquinone, acetophenone, para-toluic acid, 2-phenylethanol, trans-cinnamic acid, 4-hydroxybenzaldehyde, benzyl alcohol, benzoic acid 4-hydroxybutyl ester; Bs107T: ortho-toluic acid, benzoic acid 4-hydroxybutyl ester). The names Desulfotomaculum aquiferis sp. nov. and Desulfotomaculum profundi sp. nov. are proposed for the type strains Bs105T (=DSM 24088T=JCM 31386T) and Bs107T (=DSM 24093T=JCM 31387T).
巻・号 66(11)
ページ 4329-4338
公開日 2016-11-1
DOI 10.1099/ijsem.0.001352
PMID 27473224
MeSH Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Desulfotomaculum / classification* Desulfotomaculum / genetics Desulfotomaculum / isolation & purification Fatty Acids / chemistry France Groundwater / microbiology* Natural Gas* Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 5
リソース情報
一般微生物 JCM 31386 JCM 31387