RRC ID 49418
著者 Zachariah S, Kumari P, Das SK.
タイトル Psychrobacter pocilloporae sp. nov., isolated from a coral, Pocillopora eydouxi.
ジャーナル Int J Syst Evol Microbiol
Abstract Two closely related aerobic, Gram-stain-negative, rod-shaped bacteria (S6-60T and S6-67) were isolated from the mucus of the coral, Pocilloporaeydouxi, from the Andaman Sea, India. Heterotrophic growth on marine agar was observed at 4-37 °C and at pH 6.5-10.0; optimum growth occurred at 25-30 °C and at pH 7-9. 16S rRNA gene sequence analysis confirmed that the isolates belong to the genus Psychrobacter; the two isolates shared more than 99.5 % pairwise sequence similarity. Strain S6-60T showed a maximum 16S rRNA similarity of 98.92 % with Psychrobacter pacificensis DSM 23406T. DNA-DNA homology between the two isolates, S6-60T and S6-67, was above 90 %, whereas strain S6-60T showed less than 70 % homology with closely related type species. The DNA G+C content was 47.7 mol%. It contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipid as the major polar lipids. C10 : 0, C12 : 0 3OH, C16 : 0, C18 : 1ω9c, C17 : 1ω8c and C16 : 1ω7c were found to be the predominant fatty acids. Based on a polyphasic analysis, the isolates (S6-60T and S6-67) represent a novel species of the genus Psychrobacter for which the name Psychrobacter pocilloporae sp. nov. is proposed with S6-60T(=JCM 31058T=LMG 29157T) as the type strain.
巻・号 66(12)
ページ 5091-5098
公開日 2016-12-1
DOI 10.1099/ijsem.0.001476
PMID 27609593
MeSH Animals Anthozoa / microbiology* Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry India Nucleic Acid Hybridization Phospholipids / chemistry Phylogeny* Psychrobacter / classification* Psychrobacter / genetics Psychrobacter / isolation & purification RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 2.415
引用数 2
リソース情報
一般微生物 JCM 31058 JCM 30235