RRC ID 48683
著者 Takeya M, Hirai MY, Osanai T.
タイトル Allosteric Inhibition of Phosphoenolpyruvate Carboxylases is Determined by a Single Amino Acid Residue in Cyanobacteria.
ジャーナル Sci Rep
Abstract Phosphoenolpyruvate carboxylase (PEPC) is an important enzyme for CO2 fixation and primary metabolism in photosynthetic organisms including cyanobacteria. The kinetics and allosteric regulation of PEPCs have been studied in many organisms, but the biochemical properties of PEPC in the unicellular, non-nitrogen-fixing cyanobacterium Synechocystis sp. PCC 6803 have not been clarified. In this study, biochemical analysis revealed that the optimum pH and temperature of Synechocystis 6803 PEPC proteins were 7.3 and 30 °C, respectively. Synechocystis 6803 PEPC was found to be tolerant to allosteric inhibition by several metabolic effectors such as malate, aspartate, and fumarate compared with other cyanobacterial PEPCs. Comparative sequence and biochemical analysis showed that substitution of the glutamate residue at position 954 with lysine altered the enzyme so that it was inhibited by malate, aspartate, and fumarate. PEPC of the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 was purified, and its activity was inhibited in the presence of malate. Substitution of the lysine at position 946 (equivalent to position 954 in Synechocystis 6803) with glutamate made Anabaena 7120 PEPC tolerant to malate. These results demonstrate that the allosteric regulation of PEPC in cyanobacteria is determined by a single amino acid residue, a characteristic that is conserved in different orders.
巻・号 7
ページ 41080
公開日 2017-1-24
DOI 10.1038/srep41080
PII srep41080
PMID 28117365
PMC PMC5259782
MeSH Allosteric Regulation Amino Acid Sequence Anabaena / enzymology* Bacterial Proteins / antagonists & inhibitors Kinetics Phosphoenolpyruvate Carboxylase / antagonists & inhibitors* Phosphoenolpyruvate Carboxylase / chemistry Phosphoenolpyruvate Carboxylase / metabolism Sequence Analysis, Protein Synechocystis / enzymology*
IF 3.998
引用数 18
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
藻類 NIES-3756