RRC ID |
47770
|
著者 |
Ueta R, Abe C, Watanabe T, Sugano SS, Ishihara R, Ezura H, Osakabe Y, Osakabe K.
|
タイトル |
Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9.
|
ジャーナル |
Sci Rep
|
Abstract |
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutations effectively into SlIAA9-a key gene controlling parthenocarpy-with mutation rates of up to 100% in the T0 generation. Furthermore, analysis of off-target mutations using deep sequencing indicated that our customized gRNAs induced no additional mutations in the host genome. Regenerated mutants exhibited morphological changes in leaf shape and seedless fruit-a characteristic of parthenocarpic tomato. And the segregated next generation (T1) also showed a severe phenotype associated with the homozygous mutated genome. The system developed here could be applied to produce parthenocarpic tomato in a wide variety of cultivars, as well as other major horticultural crops, using this precise and rapid breeding technique.
|
巻・号 |
7(1)
|
ページ |
507
|
公開日 |
2017-3-30
|
DOI |
10.1038/s41598-017-00501-4
|
PII |
10.1038/s41598-017-00501-4
|
PMID |
28360425
|
PMC |
PMC5428692
|
MeSH |
Base Sequence
Breeding*
CRISPR-Cas Systems / genetics*
Gene Knockout Techniques
Mutagenesis, Site-Directed
Mutation / genetics
Parthenogenesis / genetics*
Phenotype
Plants, Genetically Modified
RNA, Guide, Kinetoplastida / genetics
Solanum lycopersicum / genetics*
Solanum lycopersicum / growth & development*
|
IF |
3.998
|
引用数 |
67
|
WOS 分野
|
PLANT SCIENCES
|
リソース情報 |
トマト |
TOMJPF00001 |