RRC ID 36894
著者 Khatib A, Arhab Y, Bentebibel A, Abousalham A, Noiriel A.
タイトル Reassessing the Potential Activities of Plant CGI-58 Protein.
ジャーナル PLoS One
Abstract Comparative Gene Identification-58 (CGI-58) is a widespread protein found in animals and plants. This protein has been shown to participate in lipolysis in mice and humans by activating Adipose triglyceride lipase (ATGL), the initial enzyme responsible for the triacylglycerol (TAG) catabolism cascade. Human mutation of CGI-58 is the cause of Chanarin-Dorfman syndrome, an orphan disease characterized by a systemic accumulation of TAG which engenders tissue disorders. The CGI-58 protein has also been shown to participate in neutral lipid metabolism in plants and, in this case, a mutation again provokes TAG accumulation. Although its roles as an ATGL coactivator and in lipid metabolism are quite clear, the catalytic activity of CGI-58 is still in question. The acyltransferase activities of CGI-58 have been speculated about, reported or even dismissed and experimental evidence that CGI-58 expressed in E. coli possesses an unambiguous catalytic activity is still lacking. To address this problem, we developed a new set of plasmids and site-directed mutants to elucidate the in vivo effects of CGI-58 expression on lipid metabolism in E. coli. By analyzing the lipid composition in selected E. coli strains expressing CGI-58 proteins, and by reinvestigating enzymatic tests with adequate controls, we show here that recombinant plant CGI-58 has none of the proposed activities previously described. Recombinant plant and mouse CGI-58 both lack acyltransferase activity towards either lysophosphatidylglycerol or lysophosphatidic acid to form phosphatidylglycerol or phosphatidic acid and recombinant plant CGI-58 does not catalyze TAG or phospholipid hydrolysis. However, expression of recombinant plant CGI-58, but not mouse CGI-58, led to a decrease in phosphatidylglycerol in all strains of E. coli tested, and a mutation of the putative catalytic residues restored a wild-type phenotype. The potential activities of plant CGI-58 are subsequently discussed.
巻・号 11(1)
ページ e0145806
公開日 2016-1-1
DOI 10.1371/journal.pone.0145806
PII PONE-D-15-33914
PMID 26745266
PMC PMC4706320
MeSH Acyltransferases / chemistry Acyltransferases / genetics Acyltransferases / metabolism* Amino Acid Sequence Animals Arabidopsis / enzymology Biocatalysis Cloning, Molecular Electrophoresis, Polyacrylamide Gel Escherichia coli / metabolism Hydrolysis Lipid Metabolism Mice Molecular Sequence Data Phenotype Phosphatidylglycerols / metabolism Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Recombinant Proteins / biosynthesis Recombinant Proteins / chemistry Recombinant Proteins / isolation & purification Sequence Alignment Sequence Homology, Amino Acid Triglycerides / metabolism
IF 2.74
引用数 6
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda10994