RRC ID 48866
著者 Tan SWS, Lee QY, Wong BSE, Cai Y, Baeg GH.
タイトル Redox Homeostasis Plays Important Roles in the Maintenance of the Drosophila Testis Germline Stem Cells.
ジャーナル Stem Cell Reports
Abstract Oxidative stress influences stem cell behavior by promoting the differentiation, proliferation, or apoptosis of stem cells. Thus, characterizing the effects of reactive oxygen species (ROS) on stem cell behavior provides insights into the significance of redox homeostasis in stem cell-associated diseases and efficient stem cell expansion for cellular therapies. We utilized the Drosophila testis as an in vivo model to examine the effects of ROS on germline stem cell (GSC) maintenance. High levels of ROS induced by alteration in Keap1/Nrf2 activity decreased GSC number by promoting precocious GSC differentiation. Notably, high ROS enhanced the transcription of the EGFR ligand spitz and the expression of phospho-Erk1/2, suggesting that high ROS-mediated GSC differentiation is through EGFR signaling. By contrast, testes with low ROS caused by Keap1 inhibition or antioxidant treatment showed an overgrowth of GSC-like cells. These findings suggest that redox homeostasis regulated by Keap1/Nrf2 signaling plays important roles in GSC maintenance.
巻・号 9(1)
ページ 342-354
公開日 2017-7-11
DOI 10.1016/j.stemcr.2017.05.034
PII S2213-6711(17)30241-2
PMID 28669604
PMC PMC5511110
MeSH Animals Cell Differentiation Cell Proliferation Drosophila Drosophila Proteins / metabolism Germ Cells / cytology* Germ Cells / metabolism Homeostasis Kelch-Like ECH-Associated Protein 1 / metabolism Male NF-E2-Related Factor 2 / metabolism Oxidation-Reduction Oxidative Stress* Reactive Oxygen Species / metabolism Signal Transduction Testis / cytology* Testis / metabolism
IF 6.032
引用数 13
WOS 分野 CELL & TISSUE ENGINEERING CELL BIOLOGY
リソース情報
ショウジョウバエ 2286R-2 2286R-3 6343R-1 6343R-2