RRC ID 47986
著者 Takada Y, Watanabe Y, Okuya K, Tatsukawa H, Hashimoto H, Hitomi K.
タイトル Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2).
ジャーナル Biosci Biotechnol Biochem
Abstract Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature. Using the recombinant protein, we biochemically characterized the enzymatic activity and also obtained a monoclonal antibody that specifically recognized OlTGT. Immunochemical analysis revealed that OlTGT was not expressed ubiquitously, unlike its mammalian orthologue, but in primarily limited tissues such as the eye, brain, spinal cord, and gas gland.
巻・号 81(3)
ページ 469-474
公開日 2017-3-1
DOI 10.1080/09168451.2016.1256757
PMID 27855535
MeSH Animals Antibodies, Monoclonal Fish Proteins / genetics Fish Proteins / immunology Fish Proteins / metabolism* GTP-Binding Proteins / chemistry* GTP-Binding Proteins / genetics GTP-Binding Proteins / immunology GTP-Binding Proteins / metabolism* Oryzias / metabolism* Protein Glutamine gamma Glutamyltransferase 2 Rats, Wistar Recombinant Proteins / genetics Recombinant Proteins / metabolism Sequence Homology, Amino Acid Transglutaminases / chemistry* Transglutaminases / genetics Transglutaminases / immunology Transglutaminases / metabolism*
IF 1.516
引用数 2
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY
リソース情報
メダカ cDNA clones