論文 - 詳細
RRC ID | 48760 |
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著者 | Kimura S, Kawano T, Iwasaki T. |
タイトル | Short polyhistidine peptides penetrate effectively into Nicotiana tabacum-cultured cells and Saccharomyces cerevisiae cells. |
ジャーナル | Biosci Biotechnol Biochem |
Abstract |
The polyhistidine peptides (PHPs) have been previously reported as novel cell-penetrating peptides and are efficiently internalized into mammal cells; however, penetration of PHPs into other cell types is unknown. In this study, the cellular uptake of PHPs in plant and yeast cells was found to be dependent on the number of histidines, and short PHPs (H6-H10 peptides) showed effective internalization. The H8 peptide showed the highest cell-penetrating capacity and localized to vacuoles in plant and yeast cells. Low-temperature conditions inhibited significantly the cellular uptake of short PHPs by both cells. However, net charge neutralization of PHPs also completely inhibited cellular uptake by plant cells, but not by yeast cells. These results indicate that short PHPs penetrate effectively into plant and yeast cells by similar mechanism with the exception of net charge dependency. The findings show the short PHPs are promising candidates for new delivery tools into plant and yeast cells. |
巻・号 | 81(1) |
ページ | 112-118 |
公開日 | 2017-1-1 |
DOI | 10.1080/09168451.2016.1234925 |
PMID | 27885926 |
MeSH | Amino Acid Sequence Animals Cell Line Cell Wall / metabolism Cell-Penetrating Peptides / chemistry* Cell-Penetrating Peptides / metabolism* Histidine / chemistry* Protein Transport Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / metabolism Structure-Activity Relationship Temperature Tobacco / cytology* Tobacco / metabolism |
IF | 1.516 |
引用数 | 3 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY |
リソース情報 | |
シロイヌナズナ / 植物培養細胞・遺伝子 | rpc00001 |