RRC ID 47613
著者 Pedrolli DB, Matern A, Wang J, Ester M, Siedler K, Breaker R, Mack M.
タイトル A highly specialized flavin mononucleotide riboswitch responds differently to similar ligands and confers roseoflavin resistance to Streptomyces davawensis.
ジャーナル Nucleic Acids Res
Abstract Streptomyces davawensis is the only organism known to synthesize the antibiotic roseoflavin, a riboflavin (vitamin B2) analog. Roseoflavin is converted to roseoflavin mononucleotide (RoFMN) and roseoflavin adenine dinucleotide in the cytoplasm of target cells. (Ribo-)Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for the biosynthesis and transport of riboflavin. Streptomyces davawensis is roseoflavin resistant, and the closely related bacterium Streptomyces coelicolor is roseoflavin sensitive. The two bacteria served as models to investigate roseoflavin resistance of S. davawensis and to analyze the mode of action of roseoflavin in S. coelicolor. Our experiments demonstrate that the ribB FMN riboswitch of S. davawensis (in contrast to the corresponding riboswitch of S. coelicolor) is able to discriminate between the two very similar flavins FMN and RoFMN and shows opposite responses to the latter ligands.
巻・号 40(17)
ページ 8662-73
公開日 2012-9-1
DOI 10.1093/nar/gks616
PII gks616
PMID 22740651
PMC PMC3458559
MeSH Anti-Bacterial Agents / pharmacology* Aptamers, Nucleotide / metabolism Cytoplasm / metabolism Drug Resistance, Fungal Flavin Mononucleotide / metabolism Flavin-Adenine Dinucleotide / analogs & derivatives Flavin-Adenine Dinucleotide / metabolism Gene Expression Regulation, Fungal* Genome, Fungal Ligands Point Mutation Protein Biosynthesis Riboflavin / analogs & derivatives Riboflavin / metabolism Riboflavin / pharmacology Riboflavin Synthase / metabolism Riboswitch* Streptomyces / drug effects Streptomyces / enzymology Streptomyces / genetics* Streptomyces coelicolor / drug effects Streptomyces coelicolor / enzymology Streptomyces coelicolor / genetics
IF 11.502
引用数 38
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
一般微生物 JCM 4913