RRC ID 37883
著者 Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K.
タイトル Co-ordinated ocular development from human iPS cells and recovery of corneal function.
ジャーナル Nature
Abstract The eye is a complex organ with highly specialized constituent tissues derived from different primordial cell lineages. The retina, for example, develops from neuroectoderm via the optic vesicle, the corneal epithelium is descended from surface ectoderm, while the iris and collagen-rich stroma of the cornea have a neural crest origin. Recent work with pluripotent stem cells in culture has revealed a previously under-appreciated level of intrinsic cellular self-organization, with a focus on the retina and retinal cells. Moreover, we and others have demonstrated the in vitro induction of a corneal epithelial cell phenotype from pluripotent stem cells. These studies, however, have a single, tissue-specific focus and fail to reflect the complexity of whole eye development. Here we demonstrate the generation from human induced pluripotent stem cells of a self-formed ectodermal autonomous multi-zone (SEAM) of ocular cells. In some respects the concentric SEAM mimics whole-eye development because cell location within different zones is indicative of lineage, spanning the ocular surface ectoderm, lens, neuro-retina, and retinal pigment epithelium. It thus represents a promising resource for new and ongoing studies of ocular morphogenesis. The approach also has translational potential and to illustrate this we show that cells isolated from the ocular surface ectodermal zone of the SEAM can be sorted and expanded ex vivo to form a corneal epithelium that recovers function in an experimentally induced animal model of corneal blindness.
巻・号 531(7594)
ページ 376-80
公開日 2016-3-17
DOI 10.1038/nature17000
PII nature17000
PMID 26958835
MeSH Animals Cell Lineage Cornea / cytology* Cornea / growth & development* Cornea / physiology Corneal Transplantation Ectoderm / cytology Epithelial Cells / cytology Epithelium, Corneal / cytology Female Humans Induced Pluripotent Stem Cells / cytology* Lens, Crystalline / cytology Mice Morphogenesis Phenotype Rabbits Recovery of Function* Retinal Pigment Epithelium / cytology
IF 42.779
引用数 67
WOS 分野 CELL BIOLOGY
リソース情報
ヒト・動物細胞 201B7(HPS0063) 253G1(HPS0002) 454E2(HPS0077)