RRC ID 25689
著者 Ishiwata A, Ozawa M, Nagasaki H, Kato M, Noda Y, Yamaguchi T, Nosaka M, Shimizu-Sato S, Nagasaki A, Maekawa M, Hirano HY, Sato Y.
タイトル Two WUSCHEL-related homeobox genes, narrow leaf2 and narrow leaf3, control leaf width in rice.
ジャーナル Plant Cell Physiol
Abstract Leaf shape is one of the key determinants of plant architecture. Leaf shape also affects the amount of sunlight captured and influences photosynthetic efficiency; thus, it is an important agronomic trait in crop plants. Understanding the molecular mechanisms governing leaf shape is a central issue of plant developmental biology and agrobiotechnology. Here, we characterized the narrow-leaf phenotype of FL90, a linkage tester line of rice (Oryza sativa). Light and scanning electron microscopic analyses of FL90 leaves revealed defects in the development of marginal regions and a reduction in the number of longitudinal veins. The narrow-leaf phenotype of FL90 shows a two-factor recessive inheritance and is caused by the loss of function of two WUSCHEL-related homeobox genes, NAL2 and NAL3 (NAL2/3), which are duplicate genes orthologous to maize NS1 and NS2 and to Arabidopsis PRS. The overexpression of NAL2/3 in transgenic rice plants results in wider leaves containing increased numbers of veins, suggesting that NAL2/3 expression regulates leaf width. Thus, NAL2/3 can be used to modulate leaf shape and improve agronomic yield in crop plants.
巻・号 54(5)
ページ 779-92
公開日 2013-5-1
DOI 10.1093/pcp/pct032
PII pct032
PMID 23420902
MeSH Amino Acid Sequence Base Sequence Crosses, Genetic Gene Expression Regulation, Plant Gene Knockdown Techniques Genes, Duplicate / genetics Genes, Homeobox / genetics* Genes, Plant / genetics* Models, Biological Molecular Sequence Data Mutation / genetics Oryza / anatomy & histology* Oryza / genetics* Oryza / ultrastructure Phenotype Plant Leaves / anatomy & histology* Plant Leaves / genetics* Plant Leaves / ultrastructure Plant Proteins / chemistry Plant Proteins / genetics* Plant Proteins / metabolism Plant Vascular Bundle / anatomy & histology Plant Vascular Bundle / growth & development Plants, Genetically Modified RNA, Messenger / genetics RNA, Messenger / metabolism
IF 4.062
引用数 34
WOS 分野 PLANT SCIENCES CELL BIOLOGY
リソース情報
イネ
情報 Oryzabase