RRC ID 35160
著者 Lin T, Pan PY, Lai YT, Chiang KW, Hsieh HL, Wu YP, Ke JM, Lee MC, Liao SS, Shih HT, Tang CY, Yang SB, Cheng HC, Wu JT, Jan YN, Lee HH.
タイトル Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.
ジャーナル PLoS Genet
Abstract During development, certain Drosophila sensory neurons undergo dendrite pruning that selectively eliminates their dendrites but leaves the axons intact. How these neurons regulate pruning activity in the dendrites remains unknown. Here, we identify a coiled-coil protein Spindle-F (Spn-F) that is required for dendrite pruning in Drosophila sensory neurons. Spn-F acts downstream of IKK-related kinase Ik2 in the same pathway for dendrite pruning. Spn-F exhibits a punctate pattern in larval neurons, whereas these Spn-F puncta become redistributed in pupal neurons, a step that is essential for dendrite pruning. The redistribution of Spn-F from puncta in pupal neurons requires the phosphorylation of Spn-F by Ik2 kinase to decrease Spn-F self-association, and depends on the function of microtubule motor dynein complex. Spn-F is a key component to link Ik2 kinase to dynein motor complex, and the formation of Ik2/Spn-F/dynein complex is critical for Spn-F redistribution and for dendrite pruning. Our findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons.
巻・号 11(11)
ページ e1005642
公開日 2015-11-1
DOI 10.1371/journal.pgen.1005642
PII PGENETICS-D-15-00331
PMID 26540204
PMC PMC4634852
MeSH Animals Cytoplasm / metabolism Dendrites / physiology* Drosophila Proteins / metabolism Drosophila Proteins / physiology* Drosophila melanogaster Dyneins / metabolism I-kappa B Kinase / metabolism* Phosphorylation Sensory Receptor Cells / cytology*
IF 5.175
引用数 5
WOS 分野 GENETICS & HEREDITY
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