論文 - 詳細
| RRC ID | 22033 |
|---|---|
| 著者 | Xie G, Zhang H, Du G, Huang Q, Liang X, Ma J, Jiao R. |
| タイトル | Uif, a large transmembrane protein with EGF-like repeats, can antagonize Notch signaling in Drosophila. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:Notch signaling is a highly conserved pathway in multi-cellular organisms ranging from flies to humans. It controls a variety of developmental processes by stimulating the expression of its target genes in a highly specific manner both spatially and temporally. The diversity, specificity and sensitivity of the Notch signaling output are regulated at distinct levels, particularly at the level of ligand-receptor interactions. METHODOLOGY/PRINCIPAL FINDINGS:Here, we report that the Drosophila gene uninflatable (uif), which encodes a large transmembrane protein with eighteen EGF-like repeats in its extracellular domain, can antagonize the canonical Notch signaling pathway. Overexpression of Uif or ectopic expression of a neomorphic form of Uif, Uif*, causes Notch signaling defects in both the wing and the sensory organ precursors. Further experiments suggest that ectopic expression of Uif* inhibits Notch signaling in cis and acts at a step that is dependent on the extracellular domain of Notch. Our results suggest that Uif can alter the accessibility of the Notch extracellular domain to its ligands during Notch activation. CONCLUSIONS/SIGNIFICANCE:Our study shows that Uif can modulate Notch activity, illustrating the importance of a delicate regulation of this signaling pathway for normal patterning. |
| 巻・号 | 7(4) |
| ページ | e36362 |
| 公開日 | 2012-4-30 |
| DOI | 10.1371/journal.pone.0036362 |
| PII | PONE-D-12-06591 |
| 解説 | PMC3340373 |
| PMID | 22558447 |
| PMC | PMC3340373 |
| MeSH | Animals Drosophila Proteins / chemistry* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism Epidermal Growth Factor / chemistry* Extracellular Matrix / metabolism Gene Expression Regulation Membrane Proteins / chemistry* Membrane Proteins / genetics Membrane Proteins / metabolism* Protein Structure, Tertiary Receptors, Notch / chemistry Receptors, Notch / metabolism* Repetitive Sequences, Amino Acid* Signal Transduction* |
| IF | 2.74 |
| 引用数 | 13 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | GS11655 (DGRC#203493) |