RRC ID 35597
著者 Moriguchi K, Yamamoto S, Tanaka K, Kurata N, Suzuki K.
タイトル Trans-kingdom horizontal DNA transfer from bacteria to yeast is highly plastic due to natural polymorphisms in auxiliary nonessential recipient genes.
ジャーナル PLoS One
Abstract With the rapid accumulation of genomic information from various eukaryotes in the last decade, genes proposed to have been derived from recent horizontal gene transfer (HGT) events have been reported even in non-phagotrophic unicellular and multicellular organisms, but the molecular pathways underlying HGT remain to be explained. The development of in vitro HGT detection systems, which permit the molecular and genetic analyses of donor and recipient organisms and quantify HGT, are helpful in order to gain insight into mechanisms that may contribute to contemporary HGT events or may have contributed to past HGT events. We applied a horizontal DNA transfer system model based on conjugal gene transfer called trans-kingdom conjugation (TKC) from the prokaryote Escherichia coli to the eukaryote Saccharomyces cerevisiae, and assessed whether and to what extent genetic variations in the eukaryotic recipient affect its receptivity to TKC. Strains from a collection of 4,823 knock-out mutants of S. cerevisiae MAT-α haploids were tested for their individual TKC receptivity. Two types of mutants, an ssd1 mutant and respiratory mutants, which are also found in experimental strains and in nature widely, were identified as highly receptive mutants. The TKC efficiency for spontaneously accrued petite (rho (-/0)) mutants of the functional allele (SSD1-V) strain showed increased receptivity. The TKC efficiency of the ssd1Δ mutant was 36% for bacterial conjugation, while that of the petite/ssd1Δ double mutants was even higher (220% in average) compared to bacterial conjugation. This increased TKC receptivity was also observed when other conjugal transfer systems were applied and the donor bacterium was changed to Agrobacterium tumefaciens. These results support the idea that the genomes of certain eukaryotes have been exposed to exogenous DNA more frequently and continuously than previously thought.
巻・号 8(9)
ページ e74590
公開日 2013-1-1
DOI 10.1371/journal.pone.0074590
PII PONE-D-13-24267
PMID 24058593
PMC PMC3772842
MeSH Conjugation, Genetic / genetics DNA, Bacterial / genetics Escherichia coli / genetics* Gene Transfer, Horizontal / genetics* Genes, Bacterial / genetics* Genetic Complementation Test Genetic Loci / genetics Genetic Testing Mitochondria / metabolism Models, Genetic Mutation / genetics Phenotype Polymorphism, Genetic* Saccharomyces cerevisiae / genetics*
IF 2.74
引用数 9
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) ME8568(HB101) CV-63(SY327(Lambdapir))
酵母 BYP7574 BYP7575 BYP7576 BYP7577