RRC ID 27489
著者 Kato S, Chan C, Itoh T, Ohkuma M.
タイトル Functional gene analysis of freshwater iron-rich flocs at circumneutral pH and isolation of a stalk-forming microaerophilic iron-oxidizing bacterium.
ジャーナル Appl Environ Microbiol
Abstract Iron-rich flocs often occur where anoxic water containing ferrous iron encounters oxygenated environments. Culture-independent molecular analyses have revealed the presence of 16S rRNA gene sequences related to diverse bacteria, including autotrophic iron oxidizers and methanotrophs in iron-rich flocs; however, the metabolic functions of the microbial communities remain poorly characterized, particularly regarding carbon cycling. In the present study, we cultivated iron-oxidizing bacteria (FeOB) and performed clone library analyses of functional genes related to carbon fixation and methane oxidization (cbbM and pmoA, respectively), in addition to bacterial and archaeal 16S rRNA genes, in freshwater iron-rich flocs at groundwater discharge points. The analyses of 16S rRNA, cbbM, and pmoA genes strongly suggested the coexistence of autotrophic iron oxidizers and methanotrophs in the flocs. Furthermore, a novel stalk-forming microaerophilic FeOB, strain OYT1, was isolated and characterized phylogenetically and physiologically. The 16S rRNA and cbbM gene sequences of OYT1 are related to those of other microaerophilic FeOB in the family Gallionellaceae, of the Betaproteobacteria, isolated from freshwater environments at circumneutral pH. The physiological characteristics of OYT1 will help elucidate the ecophysiology of microaerophilic FeOB. Overall, this study demonstrates functional roles of microorganisms in iron flocs, suggesting several possible linkages between Fe and C cycling.
巻・号 79(17)
ページ 5283-90
公開日 2013-9-1
DOI 10.1128/AEM.03840-12
PII AEM.03840-12
PMID 23811518
PMC PMC3753938
MeSH Archaeal Proteins / genetics Bacterial Proteins / genetics DNA, Archaeal / chemistry DNA, Archaeal / genetics DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fresh Water / microbiology* Gallionellaceae / classification* Gallionellaceae / genetics Gallionellaceae / isolation & purification* Hydrogen-Ion Concentration Iron / metabolism* Microbial Consortia* Molecular Sequence Data Oxidation-Reduction RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA
IF 4.016
引用数 22
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 18545