RRC ID 29414
著者 O'Connell KJ, O'Connell Motherway M, O'Callaghan J, Fitzgerald GF, Ross RP, Ventura M, Stanton C, van Sinderen D.
タイトル Metabolism of four α-glycosidic linkage-containing oligosaccharides by Bifidobacterium breve UCC2003.
ジャーナル Appl Environ Microbiol
Abstract Members of the genus Bifidobacterium are common inhabitants of the gastrointestinal tracts of humans and other mammals, where they ferment many diet-derived carbohydrates that cannot be digested by their hosts. To extend our understanding of bifidobacterial carbohydrate utilization, we investigated the molecular mechanisms by which 11 strains of Bifidobacterium breve metabolize four distinct α-glucose- and/or α-galactose-containing oligosaccharides, namely, raffinose, stachyose, melibiose, and melezitose. Here we demonstrate that all B. breve strains examined possess the ability to utilize raffinose, stachyose, and melibiose. However, the ability to metabolize melezitose was not common to all B. breve strains tested. Transcriptomic and functional genomic approaches identified a gene cluster dedicated to the metabolism of α-galactose-containing carbohydrates, while an adjacent gene cluster, dedicated to the metabolism of α-glucose-containing melezitose, was identified in strains that are able to use this carbohydrate.
巻・号 79(20)
ページ 6280-92
公開日 2013-10-1
DOI 10.1128/AEM.01775-13
PII AEM.01775-13
PMID 23913435
PMC PMC3811189
MeSH Bifidobacterium / isolation & purification Bifidobacterium / metabolism* Gene Expression Profiling Humans Melibiose / metabolism* Metabolic Networks and Pathways / genetics Multigene Family Oligosaccharides / metabolism* Raffinose / metabolism*
IF 4.016
引用数 31
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 7017 JCM 7019