RRC ID 50956
著者 Gotoh A, Nara M, Sugiyama Y, Sakanaka M, Yachi H, Kitakata A, Nakagawa A, Minami H, Okuda S, Katoh T, Katayama T, Kurihara S.
タイトル Use of Gifu Anaerobic Medium for culturing 32 dominant species of human gut microbes and its evaluation based on short-chain fatty acids fermentation profiles.
ジャーナル Biosci Biotechnol Biochem
Abstract Recently, a "human gut microbial gene catalogue," which ranks the dominance of microbe genus/species in human fecal samples, was published. Most of the bacteria ranked in the catalog are currently publicly available; however, the growth media recommended by the distributors vary among species, hampering physiological comparisons among the bacteria. To address this problem, we evaluated Gifu anaerobic medium (GAM) as a standard medium. Forty-four publicly available species of the top 56 species listed in the "human gut microbial gene catalogue" were cultured in GAM, and out of these, 32 (72%) were successfully cultured. Short-chain fatty acids from the bacterial culture supernatants were then quantified, and bacterial metabolic pathways were predicted based on in silico genomic sequence analysis. Our system provides a useful platform for assessing growth properties and analyzing metabolites of dominant human gut bacteria grown in GAM and supplemented with compounds of interest.
巻・号 81(10)
ページ 2009-2017
公開日 2017-10-1
DOI 10.1080/09168451.2017.1359486
PMID 28782454
MeSH Anaerobiosis Bacteria / genetics Bacteria / growth & development* Bacteria / metabolism* Computer Simulation Culture Techniques DNA, Bacterial / genetics Fatty Acids, Volatile / metabolism* Fermentation* Gastrointestinal Microbiome* Genomics
IF 1.516
引用数 8
リソース情報
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