RRC ID 49412
著者 Iino T, Ohkuma M, Kamagata Y, Amachi S.
タイトル Iodidimonas muriae gen. nov., sp. nov., an aerobic iodide-oxidizing bacterium isolated from brine of a natural gas and iodine recovery facility, and proposals of Iodidimonadaceae fam. nov., Iodidimonadales ord. nov., Emcibacteraceae fam. nov. and Emcibacterales ord. nov.
ジャーナル Int J Syst Evol Microbiol
Abstract A chemo-organotrophic iodide (I-)-oxidizing bacterial strain, C-3T, isolated from natural gas brine of an iodine recovery facility in Kujukuri, Chiba, Japan, was characterized for representation of a novel species in the class Alphaproteobacteria. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the nearest neighbours of strain C-3T were members of the genera Eilatimonas, Kordiimonas, Rhodothalassium and Temperatibacter with 88-91 % sequence similarity. Cells of strain C-3T were aerobic, Gram-staining-negative, non-sporulating and rod-shaped (1.3-3.6 µm in length). Strain C-3T grew optimally at 30 °C, pH 7.5 and with 3 % NaCl (w/v). Iodide oxidation to form molecular iodine (I2) was a unique trait for strain C-3T, whereas the strain did not utilize iodide as a sole electron donor for chemolithoautotrophic growth. The major isoprenoid quinone was Q-10. The major cellular fatty acids were C18 : 1ω7c and C16 : 1ω5c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and unidentified aminolipids. The G+C content of the genomic DNA was 58.5 mol%. Iodide oxidation and the major cellular fatty acids composition distinguished strain C-3T from phylogenetically related bacteria. On the basis of the phenotypic features and the phylogenetic position, a novel genus and species are proposed for strain C-3T (=JCM 17843T=LMG 28660T), to be named Iodidimonas muriae gen. nov., sp. nov. We also propose to place the distinct sublineages of the genera Iodidimonasgen. nov. and Emcibacter in the orders Iodidimonadales ord. nov. and Emcibacterales ord. nov., respectively, because these genera are located far apart from the order Kordiimonadales and form the distinct lineage in the class Alphaproteobacteria.
巻・号 66(12)
ページ 5016-5022
公開日 2016-12-1
DOI 10.1099/ijsem.0.001462
PMID 27566239
MeSH Alphaproteobacteria / classification* Alphaproteobacteria / genetics Alphaproteobacteria / isolation & purification Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Fatty Acids / chemistry Iodides / metabolism* Iodine / chemistry* Japan Natural Gas / microbiology* Phospholipids / chemistry Phylogeny* RNA, Ribosomal, 16S / genetics Salts / chemistry* Sequence Analysis, DNA
IF 2.415
引用数 6
リソース情報
一般微生物 JCM 17843 JCM 30196 JCM 17742 JCM 16665 JCM 12864 JCM 16261 JCM 30871