RRC ID 49427
著者 Sun P, Gao JL, Mao XJ, Zhao XH, Sun JG, Lu M.
タイトル Lentibacillus populi sp. nov., a moderately halophilic, endophytic bacterium isolated from a poplar tree, and emended description of the genus Lentibacillus.
ジャーナル Int J Syst Evol Microbiol
Abstract A Gram-staining-positive, moderately halophilic, aerobic, endospore-forming, rod-shaped bacterial strain, designated WD4L-1T was isolated from surface-sterilized stem tissue of a poplar tree planted in the Wudalianchi National Geopark of Heilongjiang province, PR China. This novel isolate grew in the presence of 0-15 % (w/v) NaCl, at pH 6.0-9.0 and 15-50 °C; optimum growth was observed with 7-8 % (w/v) NaCl, at pH 7.0 and 30 °C. The 16S rRNA gene sequence analysis indicated that the strain WD4L-1T belonged to the genus Lentibacillus, and was most closely related to Lentibacillus garicola SL-MJ1T with a sequence similarity of 96.1 %. The DNA G+C content of strain WD4L-1T was determined to be 36.9 mol%. The respiratory quinone was identified as menaquinone-7 (MK-7) and the major lipids were diphosphatidylglycerol and phosphatidylglycerol and one unidentified phospholipid. The major fatty acids of strain WD4L-1T were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0. The results of the physiological and biochemical tests and the minor differences in the fatty acid profiles allowed a clear phenotypic differentiation of strain WD4L-1T from the closely related Lentibacillus garicola JCM 30131T. Thus, strain WD4L-1T represents a novel species of the genus Lentibacillus, for which the name Lentibacillus populi sp. nov. is proposed. The type strain is WD4L-1T (=CGMCC 1.15454T=DSM 101738T). An emended description of the genus Lentibacillus is also provided.
巻・号 66(12)
ページ 5281-5287
公開日 2016-12-1
DOI 10.1099/ijsem.0.001508
PMID 27652598
MeSH Bacillaceae / classification* Bacillaceae / genetics Bacillaceae / isolation & purification Bacterial Typing Techniques Base Composition China DNA, Bacterial / genetics Fatty Acids / chemistry Phospholipids / chemistry Phylogeny* Populus / microbiology* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Trees / microbiology Vitamin K 2 / analogs & derivatives Vitamin K 2 / chemistry
IF 2.415
引用数 6
リソース情報
一般微生物 JCM 30131 JCM 15395