RRC ID 20966
著者 Chen CL, Wang Y, Sesaki H, Iijima M.
タイトル Myosin I links PIP3 signaling to remodeling of the actin cytoskeleton in chemotaxis.
ジャーナル Sci Signal
Abstract Class I myosins participate in various interactions between the cell membrane and the cytoskeleton. Several class I myosins preferentially bind to acidic phospholipids, such as phosphatidylserine and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], through a tail homology 1 (TH1) domain. Here, we show that the second messenger lipid phosphatidylinositol 3,4,5-trisphosphate (PIP3) binds to the TH1 domain of a subset of Dictyostelium class I myosins (ID, IE, and IF) and recruits them to the plasma membrane. The PIP3-regulated membrane recruitment of myosin I promoted chemotaxis and induced chemoattractant-stimulated actin polymerization. Similarly, PIP3 recruited human myosin IF to the plasma membrane upon chemotactic stimulation in a neutrophil cell line. These data suggest a mechanism through which the PIP3 signal is transmitted through myosin I to the actin cytoskeleton.
巻・号 5(209)
ページ ra10
公開日 2012-1-31
DOI 10.1126/scisignal.2002446
PII 5/209/ra10
PMID 22296834
PMC PMC3381520
MeSH Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism* Cell Line Cell Membrane / genetics Cell Membrane / metabolism Chemotaxis / physiology* Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* HL-60 Cells Humans Myosin Type I / genetics Myosin Type I / metabolism* Neutrophils / cytology Neutrophils / metabolism Phosphatidylinositol 4,5-Diphosphate / genetics Phosphatidylinositol 4,5-Diphosphate / metabolism Phosphatidylinositol Phosphates / genetics Phosphatidylinositol Phosphates / metabolism* Protein Structure, Tertiary Protozoan Proteins / genetics Protozoan Proteins / metabolism* Signal Transduction / physiology*
IF 6.467
引用数 47
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 G01887