論文 - 詳細
RRC ID | 20966 |
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著者 | Chen CL, Wang Y, Sesaki H, Iijima M. |
タイトル | Myosin I links PIP3 signaling to remodeling of the actin cytoskeleton in chemotaxis. |
ジャーナル | Sci Signal |
Abstract |
Class I myosins participate in various interactions between the cell membrane and the cytoskeleton. Several class I myosins preferentially bind to acidic phospholipids, such as phosphatidylserine and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], through a tail homology 1 (TH1) domain. Here, we show that the second messenger lipid phosphatidylinositol 3,4,5-trisphosphate (PIP3) binds to the TH1 domain of a subset of Dictyostelium class I myosins (ID, IE, and IF) and recruits them to the plasma membrane. The PIP3-regulated membrane recruitment of myosin I promoted chemotaxis and induced chemoattractant-stimulated actin polymerization. Similarly, PIP3 recruited human myosin IF to the plasma membrane upon chemotactic stimulation in a neutrophil cell line. These data suggest a mechanism through which the PIP3 signal is transmitted through myosin I to the actin cytoskeleton. |
巻・号 | 5(209) |
ページ | ra10 |
公開日 | 2012-1-31 |
DOI | 10.1126/scisignal.2002446 |
PII | 5/209/ra10 |
PMID | 22296834 |
PMC | PMC3381520 |
MeSH | Actin Cytoskeleton / genetics Actin Cytoskeleton / metabolism* Cell Line Cell Membrane / genetics Cell Membrane / metabolism Chemotaxis / physiology* Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* HL-60 Cells Humans Myosin Type I / genetics Myosin Type I / metabolism* Neutrophils / cytology Neutrophils / metabolism Phosphatidylinositol 4,5-Diphosphate / genetics Phosphatidylinositol 4,5-Diphosphate / metabolism Phosphatidylinositol Phosphates / genetics Phosphatidylinositol Phosphates / metabolism* Protein Structure, Tertiary Protozoan Proteins / genetics Protozoan Proteins / metabolism* Signal Transduction / physiology* |
IF | 6.467 |
引用数 | 47 |
WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
リソース情報 | |
細胞性粘菌 | G01887 |