RRC ID 29101
著者 Nuñez-Corcuera B, Birch JL, Yamada Y, Williams JG.
タイトル Transcriptional repression by a bZIP protein regulates Dictyostelium prespore differentiation.
ジャーナル PLoS One
Abstract In response to the signaling polyketide DIF-1 DimB directly activates transcription of the ecmB gene in pstB cells; a subset of the prestalk cells that are the precursors of the basal disc. We show that the promoter of pspA, a prespore-specific gene, also contains a DimB binding site. Mutation of this site causes ectopic expression in the prestalk region and ChIP analysis shows that DIF-1 induces binding of DimB to the pspA promoter. DIF-1 represses pspA gene expression in a suspension cell assay but this repression is abrogated in a dimB null strain. These results suggest a coupled control mechanism, whereby the same DIF-DimB signaling pathway that directly activates ecmB gene expression directly represses pspA gene expression.
巻・号 7(1)
ページ e29895
公開日 2012-1-1
DOI 10.1371/journal.pone.0029895
PII PONE-D-11-08613
PMID 22253818
PMC PMC3253789
MeSH Amino Acid Sequence Animals Base Sequence Basic-Leucine Zipper Transcription Factors / chemistry Basic-Leucine Zipper Transcription Factors / genetics Basic-Leucine Zipper Transcription Factors / isolation & purification Basic-Leucine Zipper Transcription Factors / metabolism* Binding Sites Cell Differentiation* / drug effects Cell Differentiation* / genetics Chromatography, Affinity Dictyostelium / cytology* Dictyostelium / drug effects Dictyostelium / genetics* Electrophoretic Mobility Shift Assay Gene Expression Regulation / drug effects Genes, Reporter / genetics Hexanones / pharmacology Hydrocarbons, Chlorinated / pharmacology Mass Spectrometry Models, Biological Molecular Sequence Data Mutation / genetics Promoter Regions, Genetic / genetics Protein Binding / drug effects Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / isolation & purification Protozoan Proteins / metabolism* Repressor Proteins / chemistry Repressor Proteins / genetics Repressor Proteins / isolation & purification Repressor Proteins / metabolism* Spores, Protozoan / cytology Spores, Protozoan / drug effects Spores, Protozoan / genetics Transcription, Genetic* / drug effects
IF 2.74
引用数 3
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 S90379