論文 - 詳細
| RRC ID | 31013 |
|---|---|
| 著者 | Cha I, Lee SH, Jeon TJ. |
| タイトル | Chemoattractant-mediated Rap1 activation requires GPCR/G proteins. |
| ジャーナル | Mol Cells |
| Abstract |
Rap1 is rapidly activated upon chemoattractant stimulation and plays an important role in cell adhesion and cytoskeletal reorganization during chemotaxis. Here, we demonstrate that G-protein coupled receptors and G-proteins are essential for chemoattractant-mediated Rap1 activation in Dictyostelium. The rapid Rap1 activation upon cAMP chemoattractant stimulation was absent in cells lacking chemoattractant cAMP receptors cAR1/cAR3 or a subunit of the heterotrimeric G-protein complex Gα2. Loss of guanylyl cyclases GCA/SGC or a cGMP-binding protein GbpC exhibited no effect on Rap1 activation kinetics. These results suggest that Rap1, a key regulator for the regulation of cytoskeletal reorganization during cell movement, is activated through the G-protein coupled receptors cAR1/cAR3 and Gα2 proteins in a way independent of the cGMP signaling pathway. |
| 巻・号 | 30(6) |
| ページ | 563-7 |
| 公開日 | 2010-12-1 |
| DOI | 10.1007/s10059-010-0153-5 |
| PMID | 21103944 |
| MeSH | Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Carrier Proteins / metabolism Cell Adhesion Cell Movement Chemotactic Factors / metabolism* Cyclic AMP / metabolism Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* GTP-Binding Protein alpha Subunits / genetics GTP-Binding Protein alpha Subunits / metabolism GTP-Binding Proteins / metabolism* Guanylate Cyclase / genetics Guanylate Cyclase / metabolism Intracellular Signaling Peptides and Proteins / metabolism Kinetics Receptors, Cyclic AMP / genetics Receptors, Cyclic AMP / metabolism* Receptors, G-Protein-Coupled / metabolism* Shelterin Complex Signal Transduction Telomere-Binding Proteins / genetics Telomere-Binding Proteins / metabolism* |
| IF | 4.081 |
| 引用数 | 8 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 細胞性粘菌 | S90405 |