RRC ID 41020
著者 Maky MA, Ishibashi N, Zendo T, Perez RH, Doud JR, Karmi M, Sonomoto K.
タイトル Enterocin F4-9, a Novel O-Linked Glycosylated Bacteriocin.
ジャーナル Appl Environ Microbiol
Abstract Enterococcus faecalis F4-9 isolated from Egyptian salted-fermented fish produces a novel bacteriocin, termed enterocin F4-9. Enterocin F4-9 was purified from the culture supernatant by three steps, and its molecular mass was determined to be 5,516.6 Da by mass spectrometry. Amino acid and DNA sequencing showed that the propeptide consists of 67 amino acid residues, with a leader peptide containing a double glycine cleavage site to produce a 47-amino-acid mature peptide. Enterocin F4-9 is modified by two molecules of N-acetylglucosamine β-O-linked to Ser37 and Thr46. The O-linked N-acetylglucosamine moieties are essential for the antimicrobial activity of enterocin F4-9. Further analysis of the enterocin F4-9 gene cluster identified enfC, which has high sequence similarity to a glycosyltransferase. The antimicrobial activity of enterocin F4-9 covered a limited range of bacteria, including, interestingly, a Gram-negative strain, Escherichia coli JM109. Enterocin F4-9 is sensitive to protease, active at a wide pH range, and moderately resistant to heat.
巻・号 81(14)
ページ 4819-26
公開日 2015-7-1
DOI 10.1128/AEM.00940-15
PII AEM.00940-15
PMID 25956765
PMC PMC4551185
MeSH Amino Acid Sequence Bacteria / drug effects Bacteriocins / chemistry Bacteriocins / metabolism* Bacteriocins / pharmacology Base Sequence Bridged-Ring Compounds / chemistry Bridged-Ring Compounds / metabolism Bridged-Ring Compounds / pharmacology Enterococcus faecalis / chemistry Enterococcus faecalis / genetics Enterococcus faecalis / isolation & purification Enterococcus faecalis / metabolism* Fish Products / microbiology* Glycosylation Molecular Sequence Data
IF 4.016
引用数 23
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 5803 JCM 8731 JCM 5804 JCM 5885 JCM 5887 JCM 1306 JCM 1157 JCM 6124 JCM 2152 JCM 2257 JCM 1465 JCM 2504 JCM 5705 JCM 5802 JCM 2414