RRC ID 48882
著者 Wang X, Olson JR, Rasoloson D, Ellenbecker M, Bailey J, Voronina E.
タイトル Dynein light chain DLC-1 promotes localization and function of the PUF protein FBF-2 in germline progenitor cells.
ジャーナル Development
Abstract PUF family translational repressors are conserved developmental regulators, but the molecular function provided by the regions flanking the PUF RNA-binding domain is unknown. In C. elegans, the PUF proteins FBF-1 and FBF-2 support germline progenitor maintenance by repressing production of meiotic proteins and use distinct mechanisms to repress their target mRNAs. We identify dynein light chain DLC-1 as an important regulator of FBF-2 function. DLC-1 directly binds to FBF-2 outside of the RNA-binding domain and promotes FBF-2 localization and function. By contrast, DLC-1 does not interact with FBF-1 and does not contribute to FBF-1 activity. Surprisingly, we find that the contribution of DLC-1 to FBF-2 activity is independent of the dynein motor. Our findings suggest that PUF protein localization and activity are mediated by sequences flanking the RNA-binding domain that bind specific molecular partners. Furthermore, these results identify a new role for DLC-1 in post-transcriptional regulation of gene expression.
巻・号 143(24)
ページ 4643-4653
公開日 2016-12-15
DOI 10.1242/dev.140921
PII dev.140921
PMID 27864381
PMC PMC5201030
MeSH Animals Binding Sites / genetics Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Dyneins / genetics Dyneins / metabolism* Gene Expression Regulation, Developmental / genetics* Protein Binding RNA Interference RNA, Small Interfering / genetics RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Stem Cells / cytology
IF 5.611
引用数 11
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
線虫 tm3153