RRC ID 48163
著者 Oh YJ, Lee HW, Lim SK, Kwon MS, Lee J, Jang JY, Lee JH, Park HW, Nam YD, Seo MJ, Roh SW, Choi HJ.
タイトル Lentibacillus kimchii sp. nov., an extremely halophilic bacterium isolated from kimchi, a Korean fermented vegetable.
ジャーナル Antonie Van Leeuwenhoek
Abstract A Gram-positive, aerobic, non-motile and extremely halophilic bacterial strain, designated K9(T), was isolated from kimchi, a Korean fermented food. The strain was observed as endospore-forming rod-shaped cells showing oxidase and catalase activity. It was found to grow at 10.0-30.0 % (w/v) NaCl (optimum, 15.0-20.0 %), pH 7.0-8.0 (optimum, pH 7.5) and 15-40 °C (optimum, 30 °C). The polar lipids of strain K9(T) were identified as phosphatidylglycerol, three unidentified phospholipids and an unidentified glycolipid. The isoprenoid quinone was identified as menaquinone-7. The major cellular fatty acids (>20 % of the total) were found to be anteisio-C15:0 and anteisio-C17:0. The cell wall peptidoglycan composition was determined to contain meso-diaminopimelic acid. The G + C content of genomic DNA was determined to be 48.2 mol %. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the isolated strain is closely related to Lentibacillus salinarum AHS-1(T) (96.7 % sequence similarity). Based on its phenotypic, chemotaxonomic and phylogenetic data, strain K9(T) is considered to represent a novel species of the genus Lentibacillus, for which the name Lentibacillus kimchii sp. nov., is proposed. The type strain is K9(T) (=KACC 18490(T) = JCM 30234(T)).
巻・号 109(6)
ページ 869-76
公開日 2016-6-1
DOI 10.1007/s10482-016-0686-5
PII 10.1007/s10482-016-0686-5
PMID 27002961
MeSH Bacillaceae / classification* Bacillaceae / genetics Bacillaceae / isolation & purification* Bacillaceae / metabolism Bacterial Typing Techniques Cell Wall / metabolism DNA, Bacterial / genetics DNA, Ribosomal / genetics Diaminopimelic Acid / metabolism Fatty Acids / metabolism Fermentation Food Microbiology Halobacteriales / classification Halobacteriales / genetics Halobacteriales / isolation & purification Halobacteriales / metabolism Peptidoglycan / chemistry Phenotype Phospholipids / metabolism Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Vegetables / microbiology* Vitamin K 2 / analogs & derivatives Vitamin K 2 / metabolism
IF 2.674
引用数 13
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 30234