RRC ID 48165
著者 Fan J, Liu C, Ma Z, Zhou S, Li W, Li J, Chu L, Wang X, Xiang W.
タイトル Dactylosporangium solaniradicis sp. nov., a novel actinobacterium isolated from a root of tomato (Solanum lycopersicum L.).
ジャーナル Antonie Van Leeuwenhoek
Abstract A novel actinobacterium, designated strain NEAU-FJL2(T), was isolated from a tomato root (Solanum lycopersicum L.) and characterised using a polyphasic approach. Morphological and chemotaxonomic properties of strain NEAU-FJL2(T) are consistent with the description of the genus Dactylosporangium. Finger-shaped sporangia were observed to form on short sporangiophores branching from the substrate hyphae. The cell wall peptidoglycan was found to contain meso- and 3-hydroxy-diaminopimelic acids; arabinose, mannose, rhamnose and xylose were found as whole-cell sugars. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The major fatty acids were identified as iso-C16:0, C16:1 ω7c and iso-C15:0. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain NEAU-FJL2(T) belongs to the genus Dactylosporangium, with Dactylosporangium sucinum JCM 19831(T) (99.3 % 16S rRNA gene sequence similarity), Dactylosporangium darangshiense JCM 17441(T) (99.2 %), Dactylosporangium fulvum JCM 5631(T) (98.9 %) and Dactylosporangium roseum JCM 3364(T) (98.8 %) as the nearest phylogenetic relatives. However, a combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain NEAU-FJL2(T) can be distinguished from them. Consequently, it is proposed that strain NEAU-FJL2(T) represents a novel species of the genus Dactylosporangium, for which the name Dactylosporangium solaniradicis sp. nov. is proposed. The type strain is NEAU-FJL2(T) (=CGMCC 4.7302(T) = DSM 100814(T)).
巻・号 109(7)
ページ 971-7
公開日 2016-7-1
DOI 10.1007/s10482-016-0697-2
PII 10.1007/s10482-016-0697-2
PMID 27091125
MeSH Cell Wall / chemistry China DNA, Bacterial / genetics DNA, Ribosomal / genetics Diaminopimelic Acid / metabolism Fatty Acids / metabolism Micromonosporaceae / classification* Micromonosporaceae / genetics Micromonosporaceae / isolation & purification* Micromonosporaceae / metabolism Nucleic Acid Hybridization Peptidoglycan / metabolism Phenotype Phospholipids / metabolism Phylogeny Plant Roots / microbiology RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Soil Microbiology Solanum lycopersicum / microbiology*
IF 2.674
引用数 2
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 19831 JCM 17441 JCM 5631 JCM 3364