| RRC ID |
52463
|
| 著者 |
Uotani K, Fujiwara T, Yoshida A, Iwata S, Morita T, Kiyono M, Yokoo S, Kunisada T, Takeda K, Hasei J, Numoto K, Nezu Y, Yonemoto T, Ishii T, Kawai A, Ochiya T, Ozaki T.
|
| タイトル |
Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma.
|
| ジャーナル |
Sci Rep
|
| Abstract |
The lack of useful biomarkers is a crucial problem for patients with soft tissue sarcomas (STSs). Emerging evidence has suggested that circulating microRNAs (miRNAs) in body fluids have novel impact as biomarkers for patients with malignant diseases, but their significance in synovial sarcoma (SS) patients remains unknown. Initial global miRNA screening using SS patient serum and SS cell culture media identified a signature of four upregulated miRNAs. Among these candidates, miR-92b-3p secretion from SS cells was confirmed, which was embedded within tumour-derived exosomes rather than argonaute-2. Animal experiments revealed a close correlation between serum miR-92b-3p levels and tumour dynamics. Clinical relevance was validated in two independent clinical cohorts, and we subsequently identified that serum miR-92b-3p levels were significantly higher in SS patients in comparison to that in healthy individuals. Moreover, serum miR-92b-3p was robust in discriminating patients with SS from the other STS patients and reflected tumour burden in SS patients. Overall, liquid biopsy using serum miR-92b-3p expression levels may represent a novel approach for monitoring tumour dynamics of SS.
|
| 巻・号 |
7(1)
|
| ページ |
14634
|
| 公開日 |
2017-11-7
|
| DOI |
10.1038/s41598-017-12660-5
|
| PII |
10.1038/s41598-017-12660-5
|
| PMID |
29116117
|
| PMC |
PMC5676745
|
| MeSH |
Adult
Animals
Biomarkers, Tumor / blood*
Case-Control Studies
Child
Circulating MicroRNA / blood
Circulating MicroRNA / genetics*
Diagnosis, Differential
Exosomes / metabolism
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / blood
MicroRNAs / genetics*
Middle Aged
Sarcoma / blood
Sarcoma / diagnosis*
Sarcoma / genetics
Sarcoma, Experimental / blood
Sarcoma, Experimental / diagnosis*
Sarcoma, Experimental / genetics
Sarcoma, Synovial / blood
Sarcoma, Synovial / diagnosis*
Sarcoma, Synovial / genetics
Tumor Cells, Cultured
Young Adult
|
| IF |
3.998
|
| 引用数 |
19
|
| リソース情報 |
| ヒト・動物細胞 |
NMS-2(RCB2347) |
