RRC ID 52494
著者 Tabe S, Hikiji H, Ariyoshi W, Hashidate-Yoshida T, Shindou H, Shimizu T, Okinaga T, Seta Y, Tominaga K, Nishihara T.
タイトル Lysophosphatidylcholine acyltransferase 4 is involved in chondrogenic differentiation of ATDC5 cells.
ジャーナル Sci Rep
Abstract Glycerophospholipids have important structural and functional roles in cells and are the main components of cellular membranes. Glycerophospholipids are formed via the de novo pathway (Kennedy pathway) and are subsequently matured in the remodeling pathway (Lands' cycle). Lands' cycle consists of two steps: deacylation of phospholipids by phospholipases A2 and reacylation of lysophospholipids by lysophospholipid acyltransferases (LPLATs). LPLATs play key roles in the maturation and maintenance of the fatty acid composition of biomembranes, and cell differentiation. We examined whether LPLATs are involved in chondrogenic differentiation of ATDC5 cells, which can differentiate into chondrocytes. Lysophosphatidylcholine acyltransferase 4 (LPCAT4) mRNA expression and LPCAT enzymatic activity towards 18:1-, 18:2-, 20:4-, and 22:6-CoA increased in the late stage of chondrogenic differentiation, when mineralization occurred. LPCAT4 knockdown decreased mRNA and protein levels of chondrogenic markers as well as Alcian blue staining intensity and alkaline phosphatase activity in ATDC5 cells. These results suggest that LPCAT4 plays important roles during the transition of chondrocytes into hypertrophic chondrocytes and/or a mineralized phenotype.
巻・号 7(1)
ページ 16701
公開日 2017-12-1
DOI 10.1038/s41598-017-16902-4
PII 10.1038/s41598-017-16902-4
PMID 29196633
PMC PMC5711957
MeSH 1-Acylglycerol-3-Phosphate O-Acyltransferase / antagonists & inhibitors 1-Acylglycerol-3-Phosphate O-Acyltransferase / genetics 1-Acylglycerol-3-Phosphate O-Acyltransferase / metabolism* Animals Bone Morphogenetic Protein 2 / genetics Bone Morphogenetic Protein 2 / metabolism Cell Differentiation Cell Line Chondrogenesis* Core Binding Factor Alpha 1 Subunit / genetics Core Binding Factor Alpha 1 Subunit / metabolism Mice RNA Interference RNA, Small Interfering / metabolism
IF 3.998
引用数 5
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)