RRC ID 52643
著者 Koshizuka T, Kobayashi T, Ishioka K, Suzutani T.
タイトル Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases.
ジャーナル Sci Rep
Abstract Nedd4 is a family of ubiquitin E3 ligases that regulate numerous cellular processes. In this report, we showed that alpha- and beta-herpesviruses have membrane proteins that regulate the function of the Nedd4 family members. Although the homology search score was quite low, UL56 of herpes simplex virus type 1 and 2, ORF0 of varicella-zoster virus, UL42 of human cytomegalovirus, and U24 of human herpesvirus 6A, 6B, and 7 all possess at least one PPxY (PY) motif in their cytoplasmic domain, and are able to bind with Itch, a member of the Nedd4 family. These viral proteins altered the localization of Itch and decreased Itch expression in co-expressing cells. In addition, these viral proteins reduced the production of retrovirus vectors through the regulation of the Nedd4 family of proteins. U24, but not the other proteins, effectively reduced CD3ε expression on the T cell surface. These viral molecules are thought to contribute to the specific function of each virus through the regulation of Nedd4 family activity.
巻・号 8(1)
ページ 4447
公開日 2018-3-13
DOI 10.1038/s41598-018-22682-2
PII 10.1038/s41598-018-22682-2
PMID 29535361
PMC PMC5849660
MeSH Binding Sites CD3 Complex / metabolism Cell Line, Tumor HEK293 Cells HeLa Cells Herpesviridae / metabolism* Humans Nedd4 Ubiquitin Protein Ligases / metabolism* Protein Binding Protein Domains Repressor Proteins / metabolism Ubiquitin-Protein Ligases / metabolism Viral Proteins / chemistry Viral Proteins / metabolism*
IF 3.998
引用数 3
リソース情報
ヒト・動物細胞 293T(RCB2202) JM(RCB0537)