RRC ID 28089
著者 Álvarez C, García I, Moreno I, Pérez-Pérez ME, Crespo JL, Romero LC, Gotor C.
タイトル Cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile in Arabidopsis.
ジャーナル Plant Cell
Abstract In Arabidopsis thaliana, DES1 is the only identified L-Cysteine desulfhydrase located in the cytosol, and it is involved in the degradation of cysteine and the concomitant production of H(2)S in this cell compartment. Detailed characterization of the T-DNA insertion mutants des1-1 and des1-2 has provided insight into the role of sulfide metabolically generated in the cytosol as a signaling molecule. Mutations of L-CYS DESULFHYDRASE 1 (DES1) impede H(2)S generation in the Arabidopsis cytosol and strongly affect plant metabolism. Senescence-associated vacuoles are detected in mesophyll protoplasts of des1 mutants. Additionally, DES1 deficiency promotes the accumulation and lipidation of the ATG8 protein, which is associated with the process of autophagy. The transcriptional profile of the des1-1 mutant corresponds to its premature senescence and autophagy-induction phenotypes, and restoring H(2)S generation has been shown to eliminate the phenotypic defects of des1 mutants. Moreover, sulfide is able to reverse ATG8 accumulation and lipidation, even in wild-type plants when autophagy is induced by carbon starvation, suggesting a general effect of sulfide on autophagy regulation that is unrelated to sulfur or nitrogen limitation stress. Our results suggest that cysteine-generated sulfide in the cytosol negatively regulates autophagy and modulates the transcriptional profile of Arabidopsis.
巻・号 24(11)
ページ 4621-34
公開日 2012-11-1
DOI 10.1105/tpc.112.105403
PII tpc.112.105403
PMID 23144183
PMC PMC3531856
MeSH Arabidopsis / drug effects Arabidopsis / enzymology Arabidopsis / genetics* Arabidopsis / physiology Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Autophagy* Carbon / metabolism Cellular Senescence Cystathionine gamma-Lyase / genetics* Cystathionine gamma-Lyase / metabolism Cysteine / metabolism* Cytosol / metabolism Gene Expression Regulation, Plant* Hydrogen Sulfide / metabolism Hydrogen Sulfide / pharmacology* Mesophyll Cells / metabolism Mutagenesis, Insertional Nitrogen / metabolism Phenotype Signal Transduction Transcriptome Vacuoles / metabolism
IF 9.618
引用数 88
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst16025