RRC ID 36153
著者 Tsuda K, Kurata N, Ohyanagi H, Hake S.
タイトル Genome-wide study of KNOX regulatory network reveals brassinosteroid catabolic genes important for shoot meristem function in rice.
ジャーナル Plant Cell
Abstract In flowering plants, knotted1-like homeobox (KNOX) transcription factors play crucial roles in establishment and maintenance of the shoot apical meristem (SAM), from which aerial organs such as leaves, stems, and flowers initiate. We report that a rice (Oryza sativa) KNOX gene Oryza sativa homeobox1 (OSH1) represses the brassinosteroid (BR) phytohormone pathway through activation of BR catabolism genes. Inducible overexpression of OSH1 caused BR insensitivity, whereas loss of function showed a BR-overproduction phenotype. Genome-wide identification of loci bound and regulated by OSH1 revealed hormonal and transcriptional regulation as the major function of OSH1. Among these targets, BR catabolism genes CYP734A2, CYP734A4, and CYP734A6 were rapidly upregulated by OSH1 induction. Furthermore, RNA interference knockdown plants of CYP734A genes arrested growth of the SAM and mimicked some osh1 phenotypes. Thus, we suggest that local control of BR levels by KNOX genes is a key regulatory step in SAM function.
巻・号 26(9)
ページ 3488-500
公開日 2014-9-1
DOI 10.1105/tpc.114.129122
PII tpc.114.129122
PMID 25194027
PMC PMC4213158
MeSH Brassinosteroids / metabolism* Brassinosteroids / pharmacology Chromatin Immunoprecipitation Conserved Sequence Gene Expression Profiling Gene Expression Regulation, Plant / drug effects Gene Knockdown Techniques Gene Regulatory Networks* Genes, Plant* Meristem / drug effects Meristem / genetics* Mutation / genetics Oryza / drug effects Oryza / genetics* Phenotype Plant Leaves / drug effects Plant Leaves / genetics Plant Proteins / genetics Plant Proteins / metabolism
IF 9.618
引用数 53
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
イネ Induced mutation lines