RRC ID 52699
著者 Yanagita K, Nagashio R, Jiang SX, Kuchitsu Y, Hachimura K, Ichinoe M, Igawa S, Fukuda E, Goshima N, Satoh Y, Murakumo Y, Saegusa M, Sato Y.
タイトル Cytoskeleton-Associated Protein 4 Is a Novel Serodiagnostic Marker for Lung Cancer.
ジャーナル Am J Pathol
Abstract Our aim was to develop a serodiagnostic marker for lung cancer. Monoclonal antibodies were generated, and one antibody designated as KU-Lu-1, recognizing cytoskeleton-associated protein 4 (CKAP4), was studied further. To evaluate the utility of KU-Lu-1 antibody as a serodiagnostic marker for lung cancer, reverse-phase protein array analysis was performed with sera of 271 lung cancer patients and 100 healthy controls. CKAP4 was detected in lung cancer cells and tissues, and its secretion into the culture supernatant was also confirmed. The serum CKAP4 levels of lung cancer patients were significantly higher than those of healthy controls (P < 0.0001), and the area under the curve of receiver-operating characteristic curve analysis was 0.890, with 81.1% sensitivity and 86.0% specificity. Furthermore, the serum CKAP4 levels were also higher in patients with stage I adenocarcinoma or squamous cell carcinoma than in healthy controls (P < 0.0001). Serum CKAP4 levels may differentiate lung cancer patients from healthy controls, and they may be detected early even in stage I non-small cell lung cancer. Serum CKAP4 levels were also significantly higher in lung cancer patients than in healthy controls in the validation set (P < 0.0001). The present results provide evidence that CKAP4 may be a novel early serodiagnostic marker for lung cancer.
巻・号 188(6)
ページ 1328-1333
公開日 2018-6-1
DOI 10.1016/j.ajpath.2018.03.007
PII S0002-9440(17)30976-8
PMID 29751934
MeSH Adenocarcinoma / blood Adenocarcinoma / diagnosis* Biomarkers, Tumor / blood* Carcinoma, Non-Small-Cell Lung / blood Carcinoma, Non-Small-Cell Lung / diagnosis* Carcinoma, Squamous Cell / blood Carcinoma, Squamous Cell / diagnosis* Case-Control Studies Humans Lung Neoplasms / blood Lung Neoplasms / diagnosis* Membrane Proteins / blood* Prognosis Protein Array Analysis Tumor Cells, Cultured
IF 3.491
引用数 10
リソース情報
ヒト・動物細胞 RERF-LC-AI(RCB0444)