RRC ID |
21141
|
著者 |
Tsugama D, Liu H, Liu S, Takano T.
|
タイトル |
Arabidopsis heterotrimeric G protein β subunit interacts with a plasma membrane 2C-type protein phosphatase, PP2C52.
|
ジャーナル |
Biochim Biophys Acta
|
Abstract |
Heterotrimeric G proteins (Gα, Gβ, Gγ) play important roles in signal transduction among various eukaryotic species. G proteins transmit signals by regulating the activities of effector proteins, but only a few Gβ-interacting effectors have been identified in plants. Here we show by a yeast two-hybrid screen that a putative myristoylated 2C-type protein phosphatase, PP2C52, is an Arabidopsis Gβ (AGB1)-interacting partner. The interaction between AGB1 and PP2C52 was confirmed by an in vitro pull-down assay and a bimolecular fluorescence complementation assay. PP2C52 transcripts were detected in many tissues. PP2C52 was localized to the plasma membrane and a mutation in the putative myristoylation site of PP2C52 disrupted its plasma membrane localization. Our results suggest that PP2C52 interacts with AGB1 on the plasma membrane and transmits signals via dephosphorylation of other proteins.
|
巻・号 |
1823(12)
|
ページ |
2254-60
|
公開日 |
2012-12-1
|
DOI |
10.1016/j.bbamcr.2012.10.001
|
PII |
S0167-4889(12)00283-2
|
PMID |
23058975
|
MeSH |
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Blotting, Western
Cell Membrane / metabolism*
Fluorescence
GTP-Binding Protein beta Subunits / metabolism*
GTP-Binding Protein gamma Subunits / metabolism*
Genetic Complementation Test
Immunoprecipitation
Mutation / genetics
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
Phosphorylation
Protein Phosphatase 2C
Protein Processing, Post-Translational
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Subcellular Fractions
Two-Hybrid System Techniques
|
IF |
3.411
|
引用数 |
18
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
シロイヌナズナ / 植物培養細胞・遺伝子 |
pda01364
pda10876 |