RRC ID 49254
著者 Kamimura Y, Miyanaga Y, Ueda M.
タイトル Heterotrimeric G-protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis.
ジャーナル Proc Natl Acad Sci U S A
Abstract Chemotactic eukaryote cells can sense chemical gradients over a wide range of concentrations via heterotrimeric G-protein signaling; however, the underlying wide-range sensing mechanisms are only partially understood. Here we report that a novel regulator of G proteins, G protein-interacting protein 1 (Gip1), is essential for extending the chemotactic range ofDictyosteliumcells. Genetic disruption of Gip1 caused severe defects in gradient sensing and directed cell migration at high but not low concentrations of chemoattractant. Also, Gip1 was found to bind and sequester G proteins in cytosolic pools. Receptor activation induced G-protein translocation to the plasma membrane from the cytosol in a Gip1-dependent manner, causing a biased redistribution of G protein on the membrane along a chemoattractant gradient. These findings suggest that Gip1 regulates G-protein shuttling between the cytosol and the membrane to ensure the availability and biased redistribution of G protein on the membrane for receptor-mediated chemotactic signaling. This mechanism offers an explanation for the wide-range sensing seen in eukaryotic chemotaxis.
巻・号 113(16)
ページ 4356-61
公開日 2016-4-19
DOI 10.1073/pnas.1516767113
PII 1516767113
PMID 27044073
PMC PMC4843477
MeSH Cell Membrane / genetics Cell Membrane / metabolism* Chemotaxis / physiology* Dictyostelium / genetics Dictyostelium / metabolism* GTP-Binding Protein Regulators / genetics GTP-Binding Protein Regulators / metabolism* Heterotrimeric GTP-Binding Proteins / genetics Heterotrimeric GTP-Binding Proteins / metabolism* Signal Transduction / physiology*
IF 9.412
引用数 11
リソース情報
細胞性粘菌 S90686 S90685 S00342 S00251