RRC ID 50401
著者 KonDo Y, Yoda S, Mizoguchi T, Ando T, Yamaguchi J, Yamamoto K, Banno Y, Fujiwara H.
タイトル Toll ligand Spätzle3 controls melanization in the stripe pattern formation in caterpillars.
ジャーナル Proc Natl Acad Sci U S A
Abstract A stripe pattern is an aposematic or camouflage coloration often observed among various caterpillars. However, how this ecologically important pattern is formed is largely unknown. The silkworm dominant mutant Zebra (Ze) has a black stripe in the anterior margin of each dorsal segment. Here, fine linkage mapping of 3,135 larvae revealed a 63-kbp region responsible for the Ze locus, which contained three candidate genes, including the Toll ligand gene spätzle3 (spz-3). Both electroporation-mediated ectopic expression and RNAi analyses showed that, among candidate genes, only processed spz-3 induced melanin pigmentation and that Toll-8 was the candidate receptor gene of spz-3 This Toll ligand/receptor set is also involved in melanization of other mutant Striped (pS ), which has broader stripes. Additional knockdown of 5 other spz family and 10 Toll-related genes caused no drastic change in the pigmentation of either mutant, suggesting that only spz-3/Toll-8 is mainly involved in the melanization process rather than pattern formation. The downstream pigmentation gene yellow was specifically up-regulated in the striped region of the Ze mutant, but spz-3 showed no such region-specific expression. Toll signaling pathways are known to be involved in innate immunity, dorsoventral axis formation, and neurotrophic functions. This study provides direct evidence that a Toll signaling pathway is co-opted to control the melanization process and adaptive striped pattern formation in caterpillars.
巻・号 114(31)
ページ 8336-8341
公開日 2017-8-1
DOI 10.1073/pnas.1707896114
PII 1707896114
PMID 28716921
PMC PMC5547650
MeSH Amino Acid Sequence / genetics Animals Bombyx / embryology* Bombyx / genetics* Chromosome Mapping Insect Proteins / genetics* Larva / metabolism Melanins / biosynthesis* Phenotype RNA Interference RNA, Small Interfering / genetics Skin Pigmentation / genetics* Toll-Like Receptor 8 / genetics*
IF 9.412
引用数 8
リソース情報
カイコ f40 f34