RRC ID 45778
著者 Kuzmanov A, Yochem J, Fay DS.
タイトル Analysis of PHA-1 reveals a limited role in pharyngeal development and novel functions in other tissues.
ジャーナル Genetics
Abstract PHA-1 encodes a cytoplasmic protein that is required for embryonic morphogenesis and attachment of the foregut (pharynx) to the mouth (buccal capsule). Previous reports have in some cases suggested that PHA-1 is essential for the differentiation of most or all pharyngeal cell types. By performing mosaic analysis with a recently acquired pha-1 null mutation (tm3671), we found that PHA-1 is not required within most or all pharyngeal cells for their proper specification, differentiation, or function. Rather, our evidence suggests that PHA-1 acts in the arcade or anterior epithelial cells of the pharynx to promote attachment of the pharynx to the future buccal capsule. In addition, PHA-1 appears to be required in the epidermis for embryonic morphogenesis, in the excretory system for osmoregulation, and in the somatic gonad for normal ovulation and fertility. PHA-1 activity is also required within at least a subset of intestinal cells for viability. To better understand the role of PHA-1 in the epidermis, we analyzed several apical junction markers in pha-1(tm3671) homozygous embryos. PHA-1 regulates the expression of several components of two apical junction complexes including AJM-1-DLG-1/discs large complex and the classical cadherin-catenin complex, which may account for the role of PHA-1 in embryonic morphogenesis.
巻・号 198(1)
ページ 259-68
公開日 2014-9-1
DOI 10.1534/genetics.114.166876
PII genetics.114.166876
PMID 25009149
PMC PMC4174937
MeSH Animals Cadherins / genetics Cadherins / metabolism Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Catenins / genetics Catenins / metabolism Cell Differentiation Epithelial Cells / cytology Epithelial Cells / metabolism Guanylate Kinases / genetics Guanylate Kinases / metabolism Organ Specificity Pharynx / cytology Pharynx / embryology Pharynx / metabolism* Tight Junctions / metabolism
IF 4.015
引用数 4
WOS 分野 GENETICS & HEREDITY
リソース情報
線虫 tm3671