RRC ID 51449
著者 Wang E, Hunter CP.
タイトル SID-1 Functions in Multiple Roles To Support Parental RNAi in Caenorhabditis elegans.
ジャーナル Genetics
Abstract Systemic RNA interference (RNAi) in Caenorhbaditis elegans requires sid-1, sid-3, and sid-5 Injected, expressed, or ingested double-stranded RNA (dsRNA) is transported between cells, enabling RNAi in most tissues, including the germline and progeny (parental RNAi). A recent report claims that parental RNAi also requires the yolk receptor rme-2 Here, we characterize the role of the sid genes and rme-2 in parental RNAi. We identify multiple independent paths for maternal dsRNA to reach embryos and initiate RNAi. We showed previously that maternal and embryonic sid-1 contribute independently to parental RNAi. Here we demonstrate a role for embryonic sid-5, but not sid-2 or sid-3 in parental RNAi. We also find that maternal rme-2 contributes to but is not required for parental RNAi. We determine that parental RNAi by feeding occurs nearly exclusively in adults. We also introduce 5-ethynyluridine to densely internally label dsRNA, avoiding complications associated with other labeling strategies such as inhibition of normal dsRNA trafficking and separation of label and RNA. Labeling shows that yolk and dsRNA do not colocalize following endocytosis, suggesting independent uptake, and, furthermore, dsRNA appears to rapidly progress through the RAB-7 endocytosis pathway independently of sid-1 activity. Our results support the premise that although sid-1 functions in multiple roles, it alone is central and absolutely required for inheritance of silencing RNAs.
巻・号 207(2)
ページ 547-557
公開日 2017-10-1
DOI 10.1534/genetics.117.300067
PII genetics.117.300067
PMID 28751423
PMC PMC5629322
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Endocytosis Extrachromosomal Inheritance Gene Silencing Membrane Proteins / genetics Membrane Proteins / metabolism* RNA, Double-Stranded / genetics RNA, Double-Stranded / metabolism RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Receptors, LDL / genetics Receptors, LDL / metabolism Yolk Sac / metabolism
IF 4.015
引用数 11
リソース情報
線虫 tm342