RRC ID 4034
著者 Linka N, Theodoulou FL, Haslam RP, Linka M, Napier JA, Neuhaus HE, Weber AP.
タイトル Peroxisomal ATP import is essential for seedling development in Arabidopsis thaliana.
ジャーナル Plant Cell
Abstract Several recent proteomic studies of plant peroxisomes indicate that the peroxisomal matrix harbors multiple ATP-dependent enzymes and chaperones. However, it is unknown whether plant peroxisomes are able to produce ATP by substrate-level phosphorylation or whether external ATP fuels the energy-dependent reactions within peroxisomes. The existence of transport proteins that supply plant peroxisomes with energy for fatty acid oxidation and other ATP-dependent processes has not previously been demonstrated. Here, we describe two Arabidopsis thaliana genes that encode peroxisomal adenine nucleotide carriers, PNC1 and PNC2. Both proteins, when fused to enhanced yellow fluorescent protein, are targeted to peroxisomes. Complementation of a yeast mutant deficient in peroxisomal ATP import and in vitro transport assays using recombinant transporter proteins revealed that PNC1 and PNC2 catalyze the counterexchange of ATP with ADP or AMP. Transgenic Arabidopsis lines repressing both PNC genes were generated using ethanol-inducible RNA interference. A detailed analysis of these plants showed that an impaired peroxisomal ATP import inhibits fatty acid breakdown during early seedling growth and other beta-oxidation reactions, such as auxin biosynthesis. We show conclusively that PNC1 and PNC2 are essential for supplying peroxisomes with ATP, indicating that no other ATP generating systems exist inside plant peroxisomes.
巻・号 20(12)
ページ 3241-57
公開日 2008-12-1
DOI 10.1105/tpc.108.062042
PII tpc.108.062042
PMID 19073763
PMC PMC2630453
MeSH Adenosine Triphosphate / metabolism* Amino Acid Sequence Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Arabidopsis Proteins / physiology* Biological Transport / genetics Biological Transport / physiology Genetic Complementation Test Lipid Metabolism / genetics Microscopy, Fluorescence Molecular Sequence Data Peroxisomes / metabolism* Plants, Genetically Modified / genetics Plants, Genetically Modified / growth & development Plants, Genetically Modified / metabolism Reverse Transcriptase Polymerase Chain Reaction Seedlings / genetics Seedlings / growth & development* Seedlings / metabolism* Sequence Homology, Amino Acid
IF 9.618
引用数 79
WOS 分野 PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda00682